Debnath Puja, Dewangan Jayant, Tandon Divya, Govila Vivek, Sharma Mona, Kumar Virendra, Govila Smita, Rath Srikanta Kumar
Department of Periodontics, Babu Banarasi Das College of Dental Sciences, BBD University, Lucknow, Uttar Pradesh, India.
Genotoxicity Lab, Division of Toxicology & Experimental Medicine, CSIR- Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
J Oral Biol Craniofac Res. 2019 Apr-Jun;9(2):226-229. doi: 10.1016/j.jobcr.2018.04.003. Epub 2018 Apr 18.
Impaired plasminogen activator inhibitor-1 (PAI-1), controlling coagulation and the fibrinolytic system is supposed to be involved in the pathogenesis of periodontitis. This study was performed to examine the association of PAI-1 gene polymorphisms with Chronic Periodontitis (CP) and alveolar bone loss severity involved with the disease and for understanding the role of genetic contributions in disease progression.
87 volunteers were included in the study. Genomic DNA was isolated from peripheral blood, subsequently, DNA samples were subjected to polymerase chain reaction and endonuclease digestion. Direct gene sequencing were performed for all the samples to identify genotype polymorphisms (rs 11560324) in the 3' untranslated region of PAI-1 gene. For bone loss assessment full mouth IOPA was taken.
Statistical analysis showed that for SNP PAI-I in 3' UTR, genotype CC (homozygous mutant) and allele C (mutant) has a risk associated with CP, although statistically significant association was not found. An increased risk of association of disease severity with CG (heterozygous mutant) and CC (homozygous mutant) genotypes, i.e., an increased carriage rate of genotype CG and CC (homozygous mutant) was evident with the increase in the severity of CP, highlighting an increased susceptibility to CP due to this gene polymorphisms.
PAI-1 genotype has a risk association with CP and alveolar bone loss severity in North-Indian population.
纤溶酶原激活物抑制剂-1（PAI-1）功能受损，其在控制凝血和纤维蛋白溶解系统方面发挥作用，被认为与牙周炎的发病机制有关。本研究旨在探讨PAI-1基因多态性与慢性牙周炎（CP）及该疾病所涉及的牙槽骨丧失严重程度之间的关联，并了解基因因素在疾病进展中的作用。
87名志愿者纳入本研究。从外周血中分离基因组DNA，随后对DNA样本进行聚合酶链反应和内切酶消化。对所有样本进行直接基因测序，以鉴定PAI-1基因3'非翻译区的基因型多态性（rs 11560324）。通过全口牙片进行骨丧失评估。
统计分析表明，对于3'UTR中的单核苷酸多态性PAI-I，基因型CC（纯合突变型）和等位基因C（突变型）与CP存在风险关联，尽管未发现具有统计学意义的关联。疾病严重程度与CG（杂合突变型）和CC（纯合突变型）基因型的关联风险增加，即随着CP严重程度的增加，基因型CG和CC（纯合突变型）的携带率明显增加，突出了这种基因多态性导致对CP易感性增加。
在北印度人群中，PAI-1基因型与CP及牙槽骨丧失严重程度存在风险关联。
