Moatsou Golfo, Zoidou Evangelia, Choundala Evangelia, Koutsaris Konstantinos, Kopsia Olga, Thergiaki Katerina, Sakkas Lambros
Laboratory of Dairy Research, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.
Foods. 2019 Jun 11;8(6):204. doi: 10.3390/foods8060204.
This paper examines the effects of the incorporation of denatured whey proteins along with salting in NaCl/KCl brine on the characteristics and ripening of sheep milk reduced-fat (RF), semi-hard cheese. Incorporation of denatured whey proteins was carried out by: i. adding commercial microparticulated whey protein (MWP) in reduced-fat cheese milk (RFM), or ii. by 'in situ' heat-induced partial denaturation of whey proteins of reduced-fat cheese milk (RFD). The implemented cheesemaking conditions included curd washing, moderate clotting, scalding temperatures, and ripening of cheeses packed in plastic bags under vacuum at 10 °C. Full-fat cheeses (FF) were manufactured in parallel. Physicochemical composition, textural profile, and proteolysis were assessed throughout 60 days of ripening. The mean moisture, fat on dry matter (FDM), moisture on non-fat substances (MNFS), protein on dry matter (PDM), salt, and salt-in-moisture (S/M) content of the RF cheeses were 47.4%, 32.8%, 57.3%, 54.3%, 1.63%, and 3.36%, respectively; pH ≈ 5.0, a ≈ 0.977, Ca ≈ 1000 mg/100 g cheese. The MNFS of FF and RF cheeses were similar. Proteolysis indices were not affected by any of the treatments, and they were similar to the FF counterparts. The applied cheesemaking technology was adequate for the production of semi-hard reduced-fat and reduced-sodium cheeses. Ripening under packaging hindered moisture loss without impairing the evolution of proteolysis and textural parameters. The same holds true for salting in NaCl/KCl brine. The high pasteurization of cheese milk was more effective for the increase of moisture and MNFS than the addition of MWP, without exhibiting any adverse effects.
本文研究了在NaCl/KCl盐卤中加入变性乳清蛋白并进行盐渍处理对低脂(RF)半硬质绵羊奶干酪特性及成熟过程的影响。变性乳清蛋白的加入方式如下:i. 在低脂干酪乳(RFM)中添加商业微颗粒化乳清蛋白(MWP);ii. 通过对低脂干酪乳(RFD)中的乳清蛋白进行“原位”热诱导部分变性。所采用的干酪制作条件包括凝乳洗涤、适度凝固、烫煮温度以及在10℃真空条件下塑料袋包装干酪的成熟过程。同时制作了全脂干酪(FF)作为对照。在60天的成熟过程中评估了干酪的理化成分、质地特征和蛋白水解情况。RF干酪的平均水分、干物质脂肪含量(FDM)、非脂肪物质水分含量(MNFS)、干物质蛋白含量(PDM)、盐分以及水分盐分含量(S/M)分别为47.4%、32.8%、57.3%、54.3%、1.63%和3.36%;pH值约为5.0,a值约为0.977,钙含量约为1000mg/100g干酪。FF和RF干酪的MNFS相似。蛋白水解指标不受任何处理的影响,且与FF干酪相当。所应用的干酪制作工艺适合生产半硬质低脂和低钠干酪。包装成熟可抑制水分流失,同时不影响蛋白水解和质地参数的变化。在NaCl/KCl盐卤中进行盐渍处理也有同样效果。对干酪乳进行高温巴氏杀菌在增加水分和MNFS方面比添加MWP更有效,且未产生任何不利影响。