Institute for Cellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of the Saarland, D-66421 Hamburg, Germany.
Interdisciplinary Centre for Research in Biotechnology (CIPBiotec), Federal University of Pampa (UNIPAMPA), Campus São Gabriel, São Gabriel 97300-000, RS, Brazil.
Toxins (Basel). 2019 Jun 12;11(6):335. doi: 10.3390/toxins11060335.
MiDCA1, a phospholipase A (PLA) neurotoxin isolated from coral snake venom, inhibited a major component of voltage-activated potassium (Kv) currents (41 ± 3% inhibition with 1 μM toxin) in mouse cultured dorsal root ganglion (DRG) neurons. In addition, the selective Kv2.1 channel blocker guangxitoxin (GxTx-1E) and MiDCA1 competitively inhibited the outward potassium current in DRG neurons. MiDCA1 (1 µM) reversibly inhibited the Kv2.1 current by 55 ± 8.9% in a oocyte heterologous system. The toxin showed selectivity for Kv2.1 channels over all the other Kv channels tested in this study. We propose that Kv2.1 channel blockade by MiDCA1 underlies the toxin's action on acetylcholine release at mammalian neuromuscular junctions.
MiDCA1 是一种从珊瑚蛇毒液中分离出来的磷脂酶 A (PLA) 神经毒素，可抑制小鼠培养的背根神经节 (DRG) 神经元中电压激活钾 (Kv) 电流的主要成分 (1 μM 毒素抑制 41 ± 3%)。此外，选择性 Kv2.1 通道阻滞剂广息痛 (GxTx-1E) 和 MiDCA1 竞争性抑制 DRG 神经元中的外向钾电流。MiDCA1（1 μM）在卵母细胞异源系统中可逆地抑制 Kv2.1 电流 55 ± 8.9%。该毒素对 Kv2.1 通道具有选择性，优于本研究中测试的所有其他 Kv 通道。我们提出，MiDCA1 对 Kv2.1 通道的阻断是其在哺乳动物神经肌肉接头处抑制乙酰胆碱释放的作用基础。
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