用于纤溶酶原激活剂及其特异性抑制剂的微孔板免疫捕获测定法。

Microplate immunocapture assay for plasminogen activators and their specific inhibitors.

作者信息

Stephens R, Leung K C, Pöllänen J, Salonen E M, Vaheri A

机构信息

Department of Virology, University of Helsinki, Finland.

出版信息

J Immunol Methods. 1987 Dec 24;105(2):245-51. doi: 10.1016/0022-1759(87)90272-9.

DOI:10.1016/0022-1759(87)90272-9

PMID:3121752

Abstract

We report a convenient sensitive enzyme activity assay for urokinase and tissue-type plasminogen activators, based on a solid-phase microtitre plate method using readily available polyclonal antibodies. The sensitivities for urokinase (active and proenzyme) and tissue activator were better than 1 ng/ml. The specificity was very high, with no significant contribution of urokinase in tissue activator assays or vice versa. This method is particularly useful for the assay of urokinase proenzyme in samples containing inhibitors. We describe how this assay may also be used to measure specific inhibitors of plasminogen activators, making use of their rapid formation of stable complexes with solid-phase activator. Inhibitors may be assayed in samples containing proenzymes.

摘要

我们报告了一种便捷灵敏的尿激酶和组织型纤溶酶原激活剂的酶活性测定方法，该方法基于使用易于获得的多克隆抗体的固相微量滴定板法。对尿激酶（活性形式和酶原）和组织激活剂的灵敏度优于1 ng/ml。特异性非常高，在组织激活剂测定中尿激酶无显著影响，反之亦然。该方法对于含有抑制剂的样品中尿激酶原的测定特别有用。我们描述了如何利用该测定法来测量纤溶酶原激活剂的特异性抑制剂，这是利用它们与固相激活剂快速形成稳定复合物的特性。抑制剂可在含有酶原的样品中进行测定。