在化学成分明确的猪胚晚期胚胎培养基（PBM）中进行体外培养。

In Vitro Culture of Late Stage Pig Embryos in a Chemically Defined Medium, Porcine Blastocyst Medium (PBM).

作者信息

Mito Tomomi, Hoshi Hiroyoshi

机构信息

Research Institute for the Functional Peptides, Higashine, Yamagata, Japan.

出版信息

Methods Mol Biol. 2019;2006:105-113. doi: 10.1007/978-1-4939-9566-0_7.

DOI:10.1007/978-1-4939-9566-0_7

PMID:31230275

Abstract

In vitro production (IVP) of porcine preimplantation embryos is an important technique not only for basic and biomedical research purposes but also for animal biotechnology application such as transgenesis, cloning, and embryo transfer. In this chapter, we demonstrate a superior IVP procedure of porcine embryos derived from cumulus-oocyte complexes (COCs) of slaughtered pig ovaries which are cultured sequentially in different defined media. Porcine blastocyst medium (PBM) particularly designed for the late stage embryo culture could improve the potential of morulae or blastocysts to develop into hatching and hatched blastocysts with good quality.

摘要

猪植入前胚胎的体外生产（IVP）是一项重要技术，不仅用于基础和生物医学研究目的，还用于动物生物技术应用，如转基因、克隆和胚胎移植。在本章中，我们展示了一种从屠宰猪卵巢的卵丘-卵母细胞复合体（COCs）获得的猪胚胎的优质IVP程序，这些胚胎在不同的特定培养基中进行顺序培养。专门为胚胎后期培养设计的猪囊胚培养基（PBM）可以提高桑椹胚或囊胚发育成优质孵化和已孵化囊胚的潜力。