Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Khalije Fars Blvd., Pistachio Co. Street, 77175-835, Rafsanjan, 7719617996, Iran.
Naunyn Schmiedebergs Arch Pharmacol. 2019 Nov;392(11):1383-1391. doi: 10.1007/s00210-019-01678-3. Epub 2019 Jun 24.
Oleuropein, as an olive leaf extract antioxidant polyphenol, has been reported to be a free radical scavenger. This study was done to investigate the effects of oleuropein, against morphine-induced hippocampus neurotoxicity and memory impairment in rats. The Morris water maze (MWM) test was used to assess the effect of oleuropein (5, 15, and 30 mg/kg, i.p., co-administrated with morphine) on spatial learning and memory of male Wistar rats which were treated with morphine sulfate (45 mg/kg, s.c., 4 weeks). In order to evaluate the cleaved caspase-3, Bax, and Bcl2 protein expression (as biochemical markers of apoptosis) in CA1 area of hippocampus tissue, the western blot test was used. Also, to evaluate the oxidative stress status of hippocampus CA1 area tissue, the malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, and glutathione peroxidase (GPx) activity were assessed. The data showed that oleuropein treatment (15 and 30 mg/kg) improves the spatial learning and memory impairments in morphine-treated animals. Also, oleuropein treatment decreased the apoptosis and oxidative stress levels in the hippocampus CA1 area of morphine-treated rats. Oleuropein can prevent the spatial learning and memory impairments in morphine-treated rats. Molecular mechanisms underlying the observed effects could be at least partially related to the inhibition of neuronal apoptosis and oxidative stress in the hippocampus CA1 area of morphine-treated rats.
橄榄苦苷作为一种橄榄叶提取物抗氧化多酚，已被报道具有自由基清除作用。本研究旨在探讨橄榄苦苷对吗啡诱导的大鼠海马神经毒性和记忆障碍的影响。通过 Morris 水迷宫（MWM）测试评估橄榄苦苷（5、15 和 30mg/kg，腹腔注射，与吗啡同时给药）对吗啡硫酸盐（45mg/kg，皮下注射，4 周）处理的雄性 Wistar 大鼠空间学习和记忆的影响。为了评估 CA1 区海马组织中 cleaved caspase-3、Bax 和 Bcl2 蛋白表达（作为细胞凋亡的生化标志物），使用 Western blot 测试。此外，为了评估海马 CA1 区组织的氧化应激状态，评估丙二醛（MDA）水平、超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶（GPx）活性。数据显示，橄榄苦苷（15 和 30mg/kg）治疗可改善吗啡处理动物的空间学习和记忆障碍。此外，橄榄苦苷治疗降低了吗啡处理大鼠海马 CA1 区的细胞凋亡和氧化应激水平。橄榄苦苷可预防吗啡处理大鼠的空间学习和记忆障碍。观察到的作用的分子机制至少部分可能与抑制吗啡处理大鼠海马 CA1 区神经元凋亡和氧化应激有关。
