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多环芳烃菲和屈改变虹鳟心肌细胞动作电位的多种离子流。

Polycyclic Aromatic Hydrocarbons Phenanthrene and Retene Modify the Action Potential via Multiple Ion Currents in Rainbow Trout Oncorhynchus mykiss Cardiac Myocytes.

机构信息

Department of Biological and Environmental Sciences, University of Jyväskylä, Jyväskylä, Finland.

Department of Environmental and Biological Sciences, University of Eastern Finland, Joensuu, Finland.

出版信息

Environ Toxicol Chem. 2019 Oct;38(10):2145-2153. doi: 10.1002/etc.4530. Epub 2019 Aug 27.

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in aqueous environments. They affect cardiovascular development and function in fishes. The 3-ring PAH phenanthrene has recently been shown to impair cardiac excitation-contraction coupling by inhibiting Ca and K currents in marine warm-water scombrid fishes. To see if similar events take place in a boreal freshwater fish, we studied whether the PAHs phenanthrene and retene (an alkylated phenanthrene) modify the action potential (AP) via effects on Na (I ), Ca (I ), or K (I , I ) currents in the ventricular myocytes of the rainbow trout (Oncorhynchus mykiss) heart. Electrophysiological characteristics of myocytes were measured using whole-cell patch clamp. Micromolar concentrations of phenanthrene and retene modified the shape of the ventricular AP, and retene profoundly shortened the AP at low micromolar concentrations. Both PAHs increased I and reduced I and I , but retene was more potent. Neither of the PAHs had an effect on I . Our results show that phenanthrene and retene affect cardiac function in rainbow trout by a mechanism that involves multiple cardiac ion channels, and the final outcome of these changes (shortening of AP) is opposite to that observed in scombrid fishes (prolongation of AP). The results also show that retene and aryl hydrocarbon receptor (AhR) agonist have an additional mechanism of toxicity besides the previously known AhR-mediated, transcription-dependent one. Environ Toxicol Chem 2019;38:2145-2153. © 2019 SETAC.

摘要

多环芳烃(PAHs)是水环 境中普遍存在的污染物。它们影响鱼类的心血管发育和功能。最近的研究表明,三环 PAH 菲对海洋温水鲭鱼的 Ca 和 K 电流具有抑制作用,从而损害了心脏兴奋-收缩偶联。为了观察类似的事件是否发生在北方淡水鱼类中,我们研究了 PAHs 菲和苊(一种烷基化菲)是否通过对虹鳟(Oncorhynchus mykiss)心室肌细胞中的 Na(I)、Ca(I)或 K(I、I)电流的影响来改变动作电位(AP)。使用全细胞膜片钳技术测量心肌细胞的电生理特性。菲和苊的微摩尔浓度改变了心室 AP 的形状,苊在低微摩尔浓度下显着缩短了 AP。两种 PAHs 均增加了 I 和减少了 I 和 I,但苊的作用更强。两种 PAHs 均对 I 没有影响。我们的结果表明,菲和苊通过涉及多种心脏离子通道的机制影响虹鳟的心脏功能,而这些变化的最终结果(AP 缩短)与鲭鱼观察到的结果相反(AP 延长)。结果还表明,除了先前已知的芳烃受体(AhR)介导的转录依赖性毒性机制外,苊和芳烃受体(AhR)激动剂还具有另一种毒性机制。环境毒理化学 2019;38:2145-2153。©2019 SETAC。

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