墨西哥锡那罗亚州番茄中与根腐病和枯萎病相关的**种**的侵袭性及分子特征分析 （注：原文中“spp.”是“species”复数形式的缩写，翻译时这里的“**种**”可根据具体语境替换为准确的种名复数形式，但由于原文未明确具体种名，所以保留“**种**”字）
Aggressiveness and molecular characterization of spp. associated with foot rot and wilt in Tomato in Sinaloa, Mexico.
作者信息
Vega-Gutiérrez Tomás Aarón, López-Urquídez Guadalupe Alfonso, Allende-Molar Raúl, Amarillas-Bueno Luis Alfonso, Romero-Gómez Sergio de Jesús, López-Orona Carlos Alfonso
机构信息
1Facultad de Agronomía, Universidad Autónoma de Sinaloa, Carretera Culiacán-Eldorado km 17.5, C.P. 80000 Culiacán, Sinaloa Mexico.
2Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Carretera Tuxpan-Tampico S/N Colonia Universitaria, C.P. 92895 Tuxpan, Veracruz Mexico.
出版信息
3 Biotech. 2019 Jul;9(7):276. doi: 10.1007/s13205-019-1808-3. Epub 2019 Jun 20.
wilt is one of the main limiting factors for tomato production in Mexico. One thousand and fifty isolates were obtained from vascular tissues tomato plants showing wilt and yellowing symptoms in Sinaloa, Mexico. The pathogenic isolates were evaluated through phylogenetic analysis of the TEF-1α gene and ITS region, morphological markers and pathogenicity tests. Within the 15 pathogenic isolates, 7 were identified as and 8 as . Phylogenetic analysis of f. sp. and isolates confirmed that both populations are constituted by distinct phylogenetic lineages. The isolates showed differences in aggressiveness; was the most aggressive. Isolates of both complexes triggered similar aerial symptoms of yellowing and darkening of the vascular tissues in tomato plants. But only isolates triggered necrosis in the plant crowns. Morphological markers allowed differentiating isolates from distinct complexes but not differentiating between lineages.
枯萎病是墨西哥番茄生产的主要限制因素之一。从墨西哥锡那罗亚州表现出枯萎和黄化症状的番茄植株维管组织中获得了1050个分离株。通过对TEF-1α基因和ITS区域进行系统发育分析、形态学标记和致病性测试对致病分离株进行了评估。在15个致病分离株中，7个被鉴定为 ，8个被鉴定为 。对 f. sp. 和 分离株的系统发育分析证实，这两个种群均由不同的系统发育谱系组成。分离株在致病性上存在差异； 最具致病性。两个复合体的分离株在番茄植株上引发了类似的地上部黄化症状和维管组织变黑。但只有 分离株在植株冠部引发坏死。形态学标记可以区分不同复合体的分离株，但不能区分谱系。
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