从培养的Lgr5干细胞生成组织工程化肠上皮。
Generating tissue-engineered intestinal epithelium from cultured Lgr5 stem cells .
作者信息
Baimakhanov Zhassulan, Torashima Yasuhiro, Soyama Akihiko, Inoue Yusuke, Sakai Yusuke, Takatsuki Mitsuhisa, Fujita Fumihiko, Kanetaka Kengo, Kuroki Tamotsu, Eguchi Susumu
机构信息
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
出版信息
Regen Ther. 2016 Sep 9;5:46-48. doi: 10.1016/j.reth.2016.08.002. eCollection 2016 Dec.
INTRODUCTION
Generating tissue-engineered small intestine (TESI) from mature intestinal cells has been established in a mouse model. The purpose of this study was to generate TESI from Lgr5 stem cells .
METHODS
We used Lgr5-EGFP mice for intestinal crypt isolation. After seven days, cultured crypts with Lgr5 stem cells were seeded onto a biodegradable polymer and implanted into omentum of NOD/SCID mice.
RESULTS
Engineered intestinal epithelium was generated from Lgr5 stem cells after four weeks of implantation. Intestinal epithelium was immunohistochemically positive for Paneth cells, enteroendocrine cells, goblet cells, microvilli of the absorptive enterocytes and Ki67.
CONCLUSION
Our observations suggest that transplanted Lgr5 stem cells can differentiate into the intestinal epithelium with further proliferative activity.
引言
在小鼠模型中已成功利用成熟肠细胞生成组织工程小肠（TESI）。本研究的目的是利用Lgr5干细胞生成TESI。
方法
我们使用Lgr5-EGFP小鼠进行肠隐窝分离。7天后，将培养有Lgr5干细胞的隐窝接种到可生物降解聚合物上，并植入NOD/SCID小鼠的网膜。
结果
植入四周后，Lgr5干细胞生成了工程化肠上皮。肠上皮经免疫组织化学检测，潘氏细胞、肠内分泌细胞、杯状细胞、吸收性肠上皮细胞的微绒毛和Ki67均呈阳性。
结论
我们的观察结果表明，移植的Lgr5干细胞可分化为具有进一步增殖活性的肠上皮。
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