RNA干扰介导的哺乳动物细胞中内体分选转运复合体（ESCRT）的抑制作用。

RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells.

作者信息

Bowers Katherine

机构信息

Division of Biosciences, Institute of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, UK.

出版信息

Methods Mol Biol. 2019;1998:305-318. doi: 10.1007/978-1-4939-9492-2_22.

DOI:10.1007/978-1-4939-9492-2_22

PMID:31250311

Abstract

Specific depletion of proteins from cultured cells using RNA interference (RNAi) has been a useful technique in assessing protein function for many years. RNAi allows the degradation of specific, targeted mRNA, allowing the effects of protein depletion on cellular processes to be examined. Here, I present a protocol for the depletion of proteins from cultured HeLa cells and list specific reagents and considerations for targeting the endosomal sorting complexes required for transport (ESCRT).

摘要

多年来，利用RNA干扰（RNAi）从培养细胞中特异性去除蛋白质一直是评估蛋白质功能的一项有用技术。RNAi可使特定的靶向mRNA降解，从而能够研究蛋白质缺失对细胞过程的影响。在此，我介绍一种从培养的HeLa细胞中去除蛋白质的方案，并列出用于靶向运输所需的内体分选复合体（ESCRT）的特定试剂和注意事项。