用于蛋白质质量控制和表征的高通量分析光散射

High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization.

作者信息

Some Daniel, Razinkov Vladimir

机构信息

Wyatt Technology Corp., Santa Barbara, CA, USA.

Drug Product Technologies, Amgen, Inc., Thousand Oaks, CA, USA.

出版信息

Methods Mol Biol. 2019;2025:335-359. doi: 10.1007/978-1-4939-9624-7_16.

DOI:10.1007/978-1-4939-9624-7_16

PMID:31267461

Abstract

We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.

摘要

我们基于静态和动态光散射技术，对蛋白质在纯化、评估和制剂过程中的表征及质量控制的高通量技术进行综述。多角度静态光散射（MALS）与快速、小体积的超高效液相色谱尺寸排阻色谱相结合，可有效表征关键生物物理特性，而高通量微孔板形式的动态光散射（DLS）能在短时间内提供大量数据，以筛选多种条件、辅料、细胞系或候选生物治疗药物。