Marques Tamara R, Braga Mariana A, Cesar Pedro H S, Marcussi Silvana, Corrêa Angelita D
Departamento de Química, Universidade Federal de Lavras/UFLA, Campus Universitário, Caixa Postal 3037, 37200-000 Lavras, MG, Brazil.
An Acad Bras Cienc. 2019 Jul 1;91(2):e20180248. doi: 10.1590/0001-3765201920180248.
The phenolic extracts of jabuticaba skin flour (JSF) were characterized by HPLC, and evaluated for their modulating action upon phospholipases A2 and proteases of snake venom, aiming at their possible use in the treatment of the various diseases associated with the action of venom toxins. Two types of extracts were prepared from JSF: aqueous and methanolic. These extracts, evaluated at different ratios, (venom: extract, m/m), significantly inhibited the phospholipase activity induced by the venom of Bothrops moojeni and Crotalus durissus terrificus, except for Bothrops atrox venom. The greatest hemolysis inhibitory action was observed for the methanolic extract, when incubated with venoms of B. moojeni and C. durissus terrificus, with inhibitions between 21 and 100%. Thrombolysis induced by venoms of B. moojeni and C. durissus terrificus was inhibited by both extracts, ranging from 32 to 83% and 51 to 83% for the aqueous and methanolic extracts, respectively. Both extracts extended coagulation time, induced by the venoms of B. moojeni and Lachesis muta muta. Inhibitory actions are related to phenolic compounds, such as gallic, syringic and p-coumaric acids, besides catechin, epigallocatechin gallate, epicatechin; resveratrol and quercetin, present in the extracts of jabuticaba skin flour, confirming their potential for nutraceutical use.
对巴西树葡萄果皮粉（JSF）的酚类提取物进行了高效液相色谱（HPLC）表征，并评估了其对蛇毒磷脂酶A2和蛋白酶的调节作用，旨在探讨其在治疗与毒液毒素作用相关的各种疾病中的潜在用途。从JSF中制备了两种提取物：水提取物和甲醇提取物。这些提取物以不同比例（毒液：提取物，质量/质量）进行评估，除了巴西矛头蝮蛇毒外，均显著抑制了莫氏矛头蝮蛇毒和三色矛头蝮蛇毒诱导的磷脂酶活性。当与莫氏矛头蝮蛇毒和三色矛头蝮蛇毒一起孵育时，甲醇提取物表现出最大的溶血抑制作用，抑制率在21%至100%之间。两种提取物均抑制了莫氏矛头蝮蛇毒和三色矛头蝮蛇毒诱导的溶栓作用，水提取物和甲醇提取物的抑制率分别为32%至83%和51%至83%。两种提取物均延长了莫氏矛头蝮蛇毒和变色矛头蝮蛇毒诱导的凝血时间。抑制作用与酚类化合物有关，如没食子酸、丁香酸和对香豆酸，此外还有儿茶素、表没食子儿茶素没食子酸酯、表儿茶素；白藜芦醇和槲皮素，这些成分存在于巴西树葡萄果皮粉提取物中，证实了它们在营养保健品方面的应用潜力。
