Pahle T, Köhler R, Gebhardt G
Sektion Tierproduktion und Veterinärmedizin der Karl-Marx-Universität Leipzig.
Arch Tierernahr. 1987 Sep;37(9):737-43. doi: 10.1080/17450398709428244.
The final product method introduced here is based on the use of 15N L-Lysine as tracer amino acid for the determination of the protein synthesis and decomposition quotas of the total body with the flux of lysine. For this purpose it is necessary to complete the N flow and N compartments of the 3-pool model by the values of lysine content. The ascertained values of protein synthesis--8.4 g/d/kg--and protein decomposition--6.9 g/d/kg--agree very well with those determined with 15N glycine as tracer amino acid.
这里介绍的最终产物法是基于使用15N-L-赖氨酸作为示踪氨基酸,通过赖氨酸通量来测定全身蛋白质合成和分解配额。为此,有必要用赖氨酸含量的值来完善三池模型的氮流量和氮区室。所确定的蛋白质合成值——8.4克/天/千克——和蛋白质分解值——6.9克/天/千克——与用15N-甘氨酸作为示踪氨基酸测定的值非常吻合。