树突状细胞姜黄素（DNC）对乳腺癌的抑制作用与姜黄素单体比较。
Inhibitory Traits of Dendrosome Curcumin (DNC) on Breast Cancer Compared to Curcumin Single Compound.
机构信息
College of Education for Pure Sciences, Biology Department, Kerbala University, Karbala, Iraq.
Department of Biology, Tehran Central Branch, Islamic Azad University, Tehran, Iran.
出版信息
J Gastrointest Cancer. 2020 Jun;51(2):527-533. doi: 10.1007/s12029-019-00273-2.
PURPOSE
Vast therapeutic traits and very low toxicity of curcumin compound have made it and related formulations promising for treatment purposes. The aim of this study was the assessment of dendrosome curcumin (DNC) inhibitory effects on breast cancer therapy compared to single curcumin compound.
METHODS
DNC was synthesized and MCF-7 cells were prepared. The cultured cells were treated with 20 μg/ml and 25 μg/ml of DNC. Real-time quantitative PCR (RT-qPCR) was performed to measure the expression of FOXCUT and MEG3 genes. Additionally, flow cytometry was applied to measure cell death rate and apoptosis.
RESULTS
The results outlined that DNC enhanced the MEG3 gene expression significantly higher than the control. Furthermore, DNC was associated with a significant decrease in the expression of FOXCUT gene as compared to the control. The application of DNC in the MCF7 cell line enhanced cell death and reduced necrosis.
CONCLUSIONS
The rate of apoptosis (programmed cell death) was enhanced, but necrosis was decreased in treated cancer cells compared to those treated with single curcumin. Accordingly, the DNC can be applied to hinder the growth and dissemination of cancer cells as a preferred approach to reduce the complications of other strategies such as chemotherapy and radiation therapy. Indeed, we concluded that DNC enhanced the expression of MEG3, a tumor suppressor, at 25 μm dose, but reduced the expression of the FOXCUT gene, possibly via the methylation of the gene. Thereby, DNC exerted a promising tumor inhibitory growth potential for the eradication of cancer cells.
目的
姜黄素化合物具有广泛的治疗特性和极低的毒性，使其及其相关制剂有望用于治疗目的。本研究的目的是评估树状突姜黄素（DNC）对乳腺癌治疗的抑制作用与单一姜黄素化合物相比。
方法
合成 DNC，制备 MCF-7 细胞。用 20μg/ml 和 25μg/ml 的 DNC 处理培养的细胞。实时定量 PCR（RT-qPCR）用于测量 FOXCUT 和 MEG3 基因的表达。此外，应用流式细胞术测量细胞死亡率和细胞凋亡。
结果
结果表明，DNC 显著增强了 MEG3 基因的表达，明显高于对照组。此外，与对照组相比，DNC 与 FOXCUT 基因表达的显著降低相关。DNC 在 MCF7 细胞系中的应用增强了细胞死亡并减少了坏死。
结论
与单独使用姜黄素相比，处理过的癌细胞中的细胞凋亡（程序性细胞死亡）率增加，而坏死减少。因此，DNC 可用于阻止癌细胞的生长和扩散，作为减少化疗和放疗等其他策略并发症的首选方法。事实上，我们得出结论，DNC 在 25μm 剂量下增强了肿瘤抑制基因 MEG3 的表达，但可能通过基因甲基化降低了 FOXCUT 基因的表达。因此，DNC 对肿瘤具有有希望的抑制生长潜力，可用于消除癌细胞。
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