热稳定微管蛋白MAP-1与微管结合并诱导微管组装。
Heat-stable microtubule protein MAP-1 binds to microtubules and induces microtubule assembly.
作者信息
Vera J C, Rivas C I, Maccioni R B
机构信息
Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262.
出版信息
FEBS Lett. 1988 May 9;232(1):159-62. doi: 10.1016/0014-5793(88)80408-3.
The microtubule-associated proteins, MAP-1, MAP-2 and tau, have been purified from brain tissue via a new approach using a heating step directly on the homogenate, followed by selective adsorption on calmodulin-Sepharose affinity columns and gel-filtration chromatography. Our results indicate that these MAPs share common biochemical properties, including heat stability, calmodulin binding and promotion of tubulin assembly into microtubules.
微管相关蛋白MAP-1、MAP-2和tau已通过一种新方法从脑组织中纯化出来，该方法直接对匀浆进行加热步骤，随后在钙调蛋白-琼脂糖亲和柱上进行选择性吸附以及凝胶过滤色谱法。我们的结果表明，这些微管相关蛋白具有共同的生化特性，包括热稳定性、钙调蛋白结合以及促进微管蛋白组装成微管。
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