Shan Xueling, Shan Xiaomeng, Pan Tao, Dai Fanzhuo, Chen Xiaohui, Wang Wenchang, Chen Zhidong
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University.
School of Chemistry and Material Engineering, Changzhou Vocational Institute of Engineering.
Anal Sci. 2019 Dec 10;35(12):1299-1304. doi: 10.2116/analsci.19P201. Epub 2019 Jul 12.
A solid-state electrochemiluminescence (ECL) sensor for the detection of reduced glutathione (GSH) based on a g-CN/SiO modified glass carbon electrode (GCE) has been developed in this research. The g-CN, which is employed as a luminophore, is simply prepared and exhibits an excellent ECL response. Mesoporous silica hollow spheres (SiO) with a large specific surface area are introduced here to increase the loading amount of g-CN. Compared to a g-CN modified GCE, the g-CN/SiO modified GCE displays a much higher ECL intensity. A high enhancement effect on the ECL intensity of g-CN/SiO modified GCE is obtained in the presence of GSH in the electrolyte. Moreover, the enhanced ECL intensity shows a good linear relationship to the GSH concentration in the range from 1.0 × 10 to 5.0 × 10 M, with a detection limit of 2.0 × 10 M (6.1 ng/mL). Besides, the ECL sensor exhibits a good anti-interference ability and has been successfully applied in the detection of GSH in commercial samples. The proposed sensor provides a promising platform for life science.
本研究开发了一种基于g-CN/SiO修饰玻碳电极（GCE）的用于检测还原型谷胱甘肽（GSH）的固态电化学发光（ECL）传感器。用作发光体的g-CN制备简单且表现出优异的ECL响应。此处引入具有大比表面积的介孔二氧化硅空心球（SiO）以增加g-CN的负载量。与g-CN修饰的GCE相比，g-CN/SiO修饰的GCE表现出更高的ECL强度。在电解液中存在GSH的情况下，g-CN/SiO修饰的GCE的ECL强度获得了很高的增强效果。此外，增强的ECL强度与1.0×10至5.0×10 M范围内的GSH浓度呈现良好的线性关系，检测限为2.0×10 M（6.1 ng/mL）。此外，该ECL传感器具有良好的抗干扰能力，并已成功应用于商业样品中GSH的检测。所提出的传感器为生命科学提供了一个有前景的平台。
