转录因子 WRKY30 介导拟南芥对黄瓜花叶病毒的抗性。
Transcription factor WRKY30 mediates resistance to Cucumber mosaic virus in Arabidopsis.
机构信息
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan, 637009, China; Ecological Security and Protection Key Laboratory of Sichuan Province and Life Science and Technology College, Mianyang Normal University, Mianyang, Sichuan, 621000, China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China; Rice and Sorghum Institute, Sichuan Academy of Agricultural Sciences, Deyang, 618000, Sichuan, China.
出版信息
Biochem Biophys Res Commun. 2019 Sep 10;517(1):118-124. doi: 10.1016/j.bbrc.2019.07.030. Epub 2019 Jul 13.
WRKY transcription factors are key regulators in regulating abiotic or biotic stress response in Arabidopsis. Previous studies showed that WRKY30 expression was induced by oxidative stress treatment, fungal elicitor, SA and ABA. However, functions of WRKY30 on viral defense are not well studied. Here, we found that Arabidopsis WRKY DNA binding protein 30 (WRKY30) plays essential roles in regulating Cucumber mosaic virus (CMV) resistance. The expression of WRKY30 was induced by CMV infection and wrky30 mutant displayed more susceptibility (including higher oxidative damages, induced reactive oxygen species synthesis and more PSII photochemistry compromise), while WRKY30 overexpression plants (WRKY30OX) exhibited more resistance to CMV infection. Moreover BRs-induced CMV tolerance is partly dependent on WRKY30. And WRKY30 expression increased after BL treatment. All these demonstrated that WRKY30 works as a positive regulator in plant CMV resistance process.
WRKY 转录因子是调控拟南芥非生物或生物胁迫响应的关键调节因子。先前的研究表明，WRKY30 的表达受氧化应激处理、真菌诱导剂、SA 和 ABA 的诱导。然而，WRKY30 在病毒防御中的功能尚未得到很好的研究。在这里，我们发现拟南芥 WRKY DNA 结合蛋白 30（WRKY30）在调控黄瓜花叶病毒（CMV）抗性中起着重要作用。WRKY30 的表达受 CMV 感染诱导，wrky30 突变体表现出更高的易感性（包括更高的氧化损伤、诱导活性氧合成和更多的 PSII 光化学损伤），而 WRKY30 过表达植株（WRKY30OX）对 CMV 感染表现出更高的抗性。此外，BRs 诱导的 CMV 耐受性部分依赖于 WRKY30。并且在 BL 处理后 WRKY30 的表达增加。所有这些都表明 WRKY30 在植物 CMV 抗性过程中作为一个正调控因子发挥作用。
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