Beijing Vegetable Research Center (BVRC), Beijing Academy of Agricultural and Forestry Sciences, Beijing Key Laboratory of Vegetable Germplasms Improvement, National Engineering Research Center for Vegetables, Beijing 100097, China.
Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA.
Development. 2019 Jul 29;146(14):dev180166. doi: 10.1242/dev.180166.
Cucumber ( L.) is an important vegetable crop that carries on vegetative growth and reproductive growth simultaneously. Indeterminate growth is favourable for fresh market under protected environments, whereas determinate growth is preferred for pickling cucumber in the once-over mechanical harvest system. The genetic basis of determinacy is largely unknown in cucumber. In this study, map-based cloning of the locus showed that the determinate growth habit is caused by a non-synonymous SNP in is expressed in the subapical regions of the shoot apical meristem, lateral meristem and young stems. Ectopic expression of rescued the terminal flower phenotype in the mutant and delayed flowering in wild-type Knockdown of resulted in determinate growth and formation of terminal flowers in cucumber. Biochemical analyses indicated that CsTFL1 interacts with a homolog of the miRNA biogenesis gene CsNOT2a; CsNOT2a interacts with FDP. Cucumber CsFT directly interacts with CsNOT2a and CsFD, and CsFD interacts with two 14-3-3 proteins. These data suggest that CsTFL1 competes with CsFT for interaction with CsNOT2a-CsFDP to inhibit determinate growth and terminal flower formation in cucumber.
黄瓜（L.）是一种重要的蔬菜作物，同时进行营养生长和生殖生长。在保护地环境下，无限生长有利于鲜食市场，而在一次性机械收获系统中，定长生长更适合腌制黄瓜。黄瓜的定长生长习性的遗传基础在很大程度上是未知的。在这项研究中，通过基于图谱的克隆，发现 基因的一个非同义 SNP 导致了定长生长习性。 在茎尖分生组织、侧芽和幼茎的亚顶端区域表达。异位表达 可挽救 突变体的顶花表型并延迟野生型的开花。 敲低导致黄瓜定长生长和顶花形成。生化分析表明，CsTFL1 与 miRNA 生物发生基因 CsNOT2a 的同源物相互作用；CsNOT2a 与 FDP 相互作用。黄瓜 CsFT 直接与 CsNOT2a 和 CsFD 相互作用，而 CsFD 与两个 14-3-3 蛋白相互作用。这些数据表明，CsTFL1 与 CsFT 竞争与 CsNOT2a-CsFDP 的相互作用，以抑制黄瓜中的定长生长和顶花形成。
