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[多种检测人血清半乳糖基转移酶技术的比较研究]

[Comparative study of various technics for the determination of human serum galactosyltransferase].

作者信息

Bar E, Malas J P, Gauduchon P, Aubert M, Sichel F, Bedard P Y, Le Talaer J Y

机构信息

Laboratoire de Bichimie, UFR des Sciences Pharmaceutiques, Université de Caen.

出版信息

Ann Biol Clin (Paris). 1988;46(2):123-8.

PMID:3132870
Abstract

We have compared three assays for serum galactosyltransferase activity, which use ovomucoïd, asialo agalactofetuin and free N-acetylglucosamine respectively as exogenous acceptors. A very good correlation between the three assays is obtained, for the whole range of GT activity. When the methods are compared to one another, the slopes of the regression lines are similar whether the sera are collected from healthy controls, pregnant women, or women suffering from benign gynecologic diseases or ovarian neoplasms. The methods which uses free N-acetylglucosamine as acceptor is rapid, cheaper and easier to perform.

摘要

我们比较了三种检测血清半乳糖基转移酶活性的方法,它们分别使用卵类粘蛋白、去唾液酸无半乳糖胎球蛋白和游离N - 乙酰葡糖胺作为外源性受体。在整个GT活性范围内,三种检测方法之间具有很好的相关性。当将这些方法相互比较时,无论血清是从健康对照者、孕妇、患有良性妇科疾病的女性还是卵巢肿瘤患者中采集的,回归线的斜率都相似。使用游离N - 乙酰葡糖胺作为受体的方法快速、成本更低且易于操作。

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