组织培养对猪半月板外植体生物力学特性的影响。
Effects of tissue culture on the biomechanical properties of porcine meniscus explants.
作者信息
Taylor Victor, Hicks Justin, Ferguson Cristin, Willey Jeffrey, Danelson Kerry
机构信息
Department of Orthopaedic Surgery, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, United States of America; Department of Anatomy and Cell Biology, George Washington University School of Medicine and Health Sciences, 2300 I St NW, Washington, D.C. 20052, United States of America..
Department of Orthopaedic Surgery, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, United States of America; Department of Orthopaedic Surgery, Washington University, 660 S Euclid, St. Louis, MO 63110, United States of America.
出版信息
Clin Biomech (Bristol). 2019 Oct;69:120-126. doi: 10.1016/j.clinbiomech.2019.06.019. Epub 2019 Jul 7.
BACKGROUND
The meniscus is critical for the normal functioning of the knee joint. The specific aim of this study was to validate an in vitro culture model of meniscus explants for testing the impact of culture conditions on meniscus biomechanical properties. We hypothesized that culturing menisci in the presence of intermediate and high concentration of serum would have a positive effect on the compressive stiffness of the meniscus.
METHODS
Unconstrained microindentation testing was performed on porcine meniscus explants cultured with varying concentrations 1%, 5%, or 10% of fetal bovine serum media. Meniscus explants that were not cultured were used as a control. These tests quantified the Young's Modulus of the listed groups of cultured and uncultured explant tissues.
FINDINGS
The Young's modulus for 10% cultured explants were significantly higher compared to the control, 1%, and 5% cultured meniscus explants. There was no statistical significance when the Young's modulus between control, 1%, and 5% cultured explants were compared.
INTERPRETATION
These results suggest that low concentrations of serum do not impart an anabolic effect on meniscus tissue explant biomechanical properties.
背景
半月板对于膝关节的正常功能至关重要。本研究的具体目的是验证半月板外植体的体外培养模型，以测试培养条件对半月板生物力学特性的影响。我们假设在中等浓度和高浓度血清存在下培养半月板会对半月板的压缩刚度产生积极影响。
方法
对用不同浓度（1%、5%或10%）胎牛血清培养基培养的猪半月板外植体进行无约束微压痕测试。未培养的半月板外植体用作对照。这些测试量化了所列培养和未培养外植体组织组的杨氏模量。
结果
与对照、1%和5%培养的半月板外植体相比，10%培养的外植体的杨氏模量显著更高。当比较对照、1%和5%培养的外植体之间的杨氏模量时，没有统计学意义。
解读
这些结果表明，低浓度血清不会对半月板组织外植体的生物力学特性产生合成代谢作用。
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