静脉微透析导管用于氨基糖苷类和万古霉素治疗药物监测的体外评估。
In vitro evaluation of an intravenous microdialysis catheter for therapeutic drug monitoring of gentamicin and vancomycin.
机构信息
Department of Clinical Pharmacy and Pharmacology University of Groningen University Medical Center Groningen Groningen The Netherlands.
Department of Clinical Pharmacy Máxima Medical Center Veldhoven The Netherlands.
出版信息
Pharmacol Res Perspect. 2019 Jun 26;7(4):e00483. doi: 10.1002/prp2.483. eCollection 2019 Aug.
A central venous catheter with a built-in microdialysis membrane is available for continuous lactate and glucose monitoring in the intensive care unit (ICU). As this catheter might also be suitable for repeated measurements of unbound drug levels, we studied in vitro the feasibility of monitoring unbound antibiotic concentrations. The catheter was placed in various media at 37°C spiked with gentamicin or vancomycin. Dialysate fractions were repeatedly collected over 3 hours with a NaCl 0.9% perfusate flow of 5 μL/min. Total and unbound drug concentrations in medium and perfusate were measured by immunoassay. After 60 minutes stable recovery for both drugs was observed, with mean ±SD relative recoveries of vancomycin and gentamicin in human serum of 64% ±0.4% and 73% ±3%. The recoveries of the unbound concentrations were 91% ±3% and 91% ±4%. This intravenous microdialysis system may be a very useful platform for therapeutic drug monitoring in the ICU.
一种带有内置微透析膜的中心静脉导管可用于重症监护病房（ICU）中连续的乳酸和葡萄糖监测。由于该导管也可能适用于重复测量游离药物水平，我们研究了体外监测游离抗生素浓度的可行性。将导管置于 37°C 的各种介质中，加入庆大霉素或万古霉素。用 5μL/min 的 0.9%NaCl 灌注液在 3 小时内重复采集透析液部分。通过免疫测定法测量介质和灌注液中的总药物和游离药物浓度。60 分钟后，两种药物的回收率均稳定，万古霉素和庆大霉素在人血清中的相对回收率分别为 64%±0.4%和 73%±3%。游离浓度的回收率分别为 91%±3%和 91%±4%。这种静脉内微透析系统可能是 ICU 治疗药物监测的非常有用的平台。
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