Alexander M H, Lazan K S, Rasmussen K M
Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853.
J Nutr. 1988 Jul;118(7):883-7. doi: 10.1093/jn/118.7.883.
To assess the effect of chronic protein-energy malnutrition on fecundability (ovulation rate), fecundity (percentage of animals bred that conceived) and fertility (live fetuses at d 18 of pregnancy), 42-d-old female rats were assigned to six groups: two dietary treatment groups and, within each treatment group, three outcome groups. Rats were fed ad libitum (AL) or were chronically restricted (CR) to 50% of AL intake for 28 d before ovulation was assessed and then until implantation and fetal viability were measured. In the ovulation groups, corpora lutea were counted at estrus. In the implantation groups, blastocyst implantation sites were quantified by using Pontamine Blue dye at d 5 of pregnancy. In the viability groups, live and dead fetuses and metrial nodes were counted at d 18 of pregnancy. CR rats had 74% as many corpora lutea as AL rats; however, fecundity did not differ between dietary treatments. CR rats had 71% as many blastocyst implantation sites and 65% as many viable fetuses as AL rats. Chronic malnutrition negatively affected weight gain before and during pregnancy as well as reproductive performance. These findings help to explain why CR rats have fewer progeny.
为评估慢性蛋白质 - 能量营养不良对受孕能力（排卵率）、生殖力（受孕的配种动物百分比）和生育力（妊娠第18天的活胎数）的影响，将42日龄雌性大鼠分为六组：两个饮食处理组，且在每个处理组内又分为三个结果组。在评估排卵前28天，大鼠自由采食（AL）或长期限制采食（CR），使其摄入量为AL组的50%，然后持续至测量着床和胎儿活力时。在排卵组，在发情期计数黄体。在着床组，于妊娠第5天使用丽春红染料对囊胚着床部位进行定量。在活力组，在妊娠第18天计数活胎、死胎和胎盘结节。CR组大鼠的黄体数量为AL组大鼠的74%；然而，饮食处理组之间的生殖力并无差异。CR组大鼠的囊胚着床部位数量为AL组大鼠的71%，活胎数量为AL组大鼠的65%。慢性营养不良对妊娠前及妊娠期间的体重增加以及生殖性能均产生负面影响。这些发现有助于解释为什么CR组大鼠的后代较少。
