Gusev K O, Vigont V V, Grekhnev D A, Shalygin A V, Glushankova L N, Kaznacheeva E V
Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia.
Bull Exp Biol Med. 2019 Jul;167(3):311-314. doi: 10.1007/s10517-019-04516-x. Epub 2019 Jul 26.
The fluorescent dye fura-2 AM was employed to record activation of Ca entry in response to a decrease in Ca concentration in the endoplasmic reticulum. Using whole-cell voltage clamp technique, we revealed Ca currents with an amplitude of 0.46±0.13 pA/pF that passed through selective channels with current-voltage characteristics similar to those of classical store-operated CRAC channels. These currents were sensitive to 2-APB (50 μM), an inhibitor of store-operated channels. The data suggest that store-operated calcium entry is a characteristic feature of mature ventricular cardiomyocytes. Pathological alterations in store-operated Ca entry can be implicated in the development of heart diseases.
使用荧光染料fura-2 AM记录内质网中钙浓度降低时钙内流的激活情况。采用全细胞膜片钳技术，我们发现了幅度为0.46±0.13 pA/pF的钙电流，该电流通过具有与经典的储存操纵性CRAC通道相似电流-电压特性的选择性通道。这些电流对储存操纵性通道的抑制剂2-APB（50 μM）敏感。数据表明，储存操纵性钙内流是成熟心室心肌细胞的一个特征。储存操纵性钙内流的病理改变可能与心脏病的发生有关。
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