Al Qattan Duaa, Shatarat Amjad, Alzghoul Loai, Khaled Aya, Abdallah Ala, ELBeltagy Maha
Department of Pathology, The University of Jordan, Amman, Jordan.
Department of Anatomy, The University of Jordan, Amman, Jordan.
Anat Histol Embryol. 2019 Sep;48(5):437-443. doi: 10.1111/ahe.12464. Epub 2019 Jul 26.
Sexual dimorphism exists at all levels of the nervous system, from genetic, anatomical and system levels. The sexual dimorphism in the axonal content of the corpus callosum (CC) has always been controversial; hence, the aim of this study was to analyse the differences in total, myelinated and unmyelinated axons density of various regions of the CC between male and female rats. To assess that, six pairs of adult male and female rats were perfused and the CC was removed and sectioned. Four sections from different subregions of the corpus callosum that represent the genu, anterior body, posterior body, and splenium, were stained, and electron microscopic images were captured using stereological guidelines. Later, the axons density for each subregion was calculated and compared between males and females. The findings of the present study indicated region-specific differences in the myelinated, unmyelinated or the ratio of myelinated/total axons in the CC between male and female rats.
从基因、解剖学和系统水平来看，性二态性存在于神经系统的各个层面。胼胝体（CC）轴突内容物中的性二态性一直存在争议；因此，本研究的目的是分析雄性和雌性大鼠CC不同区域的总轴突、有髓轴突和无髓轴突密度的差异。为了评估这一点，对六对成年雄性和雌性大鼠进行灌注，取出CC并切片。对胼胝体不同亚区域代表膝部、前体、后体和压部的四个切片进行染色，并按照体视学指南拍摄电子显微镜图像。随后，计算每个亚区域的轴突密度，并在雄性和雌性之间进行比较。本研究结果表明，雄性和雌性大鼠CC中有髓轴突、无髓轴突或有髓/总轴突比例存在区域特异性差异。
