Southeast Dairy Foods Research Center, North Carolina State University, Raleigh 27695.
Northeast Dairy Foods Research Center, Department of Food Science, Cornell University, Ithaca, NY 14853.
J Dairy Sci. 2019 Oct;102(10):8768-8784. doi: 10.3168/jds.2019-16247. Epub 2019 Jul 24.
Acid whey resulting from the production of soft cheeses is a disposal problem for the dairy industry. Few uses have been found for acid whey because of its high ash content, low pH, and high organic acid content. The objective of this study was to explore the potential of recovery of whey protein from cottage cheese acid whey for use in yogurt. Cottage cheese acid whey and Cheddar cheese whey were produced from standard cottage cheese and Cheddar cheese-making procedures, respectively. The whey was separated and pasteurized by high temperature, short time pasteurization and stored at 4°C. Food-grade ammonium hydroxide was used to neutralize the acid whey to a pH of 6.4. The whey was heated to 50°C and concentrated using ultrafiltration and diafiltration with 11 polyethersulfone cartridge membrane filters (10,000-kDa cutoff) to 25% total solids and 80% protein. Skim milk was concentrated to 6% total protein. Nonfat, unflavored set-style yogurts (6.0 ± 0.1% protein, 15 ± 1.0% solids) were made from skim milk with added acid whey protein concentrate, skim milk with added sweet whey protein concentrate, or skim milk concentrate. Yogurt mixes were standardized to lactose and fat of 6.50% and 0.10%, respectively. Yogurt was fermented at 43°C to pH 4.6 and stored at 4°C. The experiment was replicated in triplicate. Titratable acidity, pH, whey separation, color, and gel strength were measured weekly in yogurts through 8 wk. Trained panel profiling was conducted on 0, 14, 28, and 56 d. Fat-free yogurts produced with added neutralized fresh liquid acid whey protein concentrate had flavor attributes similar those with added fresh liquid sweet whey protein but had lower gel strength attributes, which translated to differences in trained panel texture attributes and lower consumer liking scores for fat-free yogurt made with added acid whey protein ingredient. Difference in pH was the main contributor to texture differences, as higher pH in acid whey protein yogurts changed gel structure formation and water-holding capacity of the yogurt gel. In a second part of the study, the yogurt mix was reformulated to address texture differences. The reformulated yogurt mix at 2% milkfat and using a lower level of sweet and acid whey ingredient performed at parity with control yogurts in consumer sensory trials. Fresh liquid acid whey protein concentrates from cottage cheese manufacture can be used as a liquid protein ingredient source for manufacture of yogurt in the same factory.
从软奶酪生产中产生的酸性乳清是奶制品行业的一个处理难题。由于其高灰分、低 pH 值和高有机酸含量,很少有发现可以利用酸性乳清的方法。本研究的目的是探索从农家干酪酸性乳清中回收乳清蛋白并将其用于酸奶的潜力。农家奶酪酸性乳清和切达奶酪乳清分别由标准的农家奶酪和切达奶酪制作工艺制成。乳清通过高温、短时间巴氏杀菌分离并巴氏杀菌,并在 4°C 下储存。使用食品级氨水溶液将酸性乳清中和至 pH 值为 6.4。将乳清加热至 50°C,并用 11 个聚醚砜(PES)膜过滤器(10000kDa 截止)进行超滤和透析浓缩至 25%总固体和 80%蛋白质。脱脂奶粉浓缩至 6%总蛋白。从脱脂奶粉中加入酸乳清蛋白浓缩物、脱脂奶粉中加入甜乳清蛋白浓缩物或脱脂奶粉浓缩物制成非脂肪、无风味的凝固型酸奶(6.0±0.1%蛋白质,15±1.0%固体)。酸奶混合物的乳糖和脂肪标准化分别为 6.50%和 0.10%。酸奶在 43°C 发酵至 pH 值为 4.6,然后在 4°C 下储存。该实验重复了 3 次。在 8 周内每周测量酸奶的滴定酸度、pH 值、乳清分离、颜色和凝胶强度。在 0、14、28 和 56 天进行了经过培训的小组特征分析。用添加中和后的新鲜液态酸乳清蛋白浓缩物制成的无脂酸奶具有与添加新鲜液态甜乳清蛋白相似的风味属性,但凝胶强度属性较低,这转化为经过培训的小组质地属性的差异以及消费者对添加酸乳清蛋白成分的无脂酸奶的喜爱评分较低。pH 值的差异是质地差异的主要原因,因为酸乳清蛋白酸奶中的较高 pH 值改变了酸奶凝胶的凝胶结构形成和持水能力。在研究的第二部分,对酸奶混合物进行了重新配方,以解决质地差异的问题。在消费者感官试验中,使用 2%牛奶脂肪和较低水平的甜乳清和酸乳清成分的重新配方酸奶混合物与对照酸奶具有同等性能。从农家奶酪生产中获得的新鲜液态酸乳清蛋白浓缩物可作为同一工厂生产酸奶的液体蛋白质原料。