Association of gene with incomplete hemolytic phenotype in clinical .

This experiment aimed to evaluate the correlation between the hemolytic phenotype of and gene in terms of characteristics of antibiotic resistance. Two-hundred and eleven strains of hospital-acquired and their bacterial susceptibility to 20 antibiotics were determined by MicroScan WalkAway96. All strains were cultured on Columbia sheep blood agar plates for 24 hours and then underwent ten passages for investigation of their hemolytic phenotypes. produced incomplete β-hemolytic phenotype, termed as  strains with incomplete hemolytic phenotype (SIHP). The gene was identified by PCR amplification followed by DNA sequencing. Statistical analyses of the data were performed using SPSS version 16.0 software. Fifty-two (24.64%) strains were confirmed to maintain the incomplete hemolytic phenotype of (SIHP). Meanwhile, 15 (7.11%) of 211 strains were found to carry the gene, and eight of the 15 strains were SIHP. Compared with  strains with complete hemolytic phenotype (SCHP), SIHP showed higher susceptibility to seven of the 20 antibiotics (oxacillin, ciprofloxacin, gentamicin, ceftriaxone, cefoxitin, levofloxacin, and moxifloxacin) (<0.05). The -positive bacteria had a higher rate of resistance to four antibiotics (rifampin, ciprofloxacin, levofloxacin, and moxifloxacin) in comparison with the -negative strains (0.05). SIHP had a high frequency of gene. The -positive isolates showed less resistance to rifampin, ciprofloxacin, levofloxacin, and moxifloxacin. Additionally, the majority of SIHP isolates (61.54%) were methicillin-resistant . SIHP strains had significantly higher antibiotic resistance to cefoxitin when compared with SCHP, while SCHP strains had a high rate of antibiotic resistance to ciprofloxacin, gentamicin, ceftriaxone, levofloxacin, and moxifloxacin. The results may help to provide medical advice for selection of antibiotics for patients with SIHP-associated infections.