油红 O 在检测双链 DNA 中的新作用。

New role of oil red O in detection of double stranded DNA.

机构信息

State Key Laboratory of SupraMecular Structure and Materials, Institute of Theoretical Chemistry, College of Chemistry, Jilin University, 2699 Qianjin Street, 130012, Changchun, PR China.

出版信息

Talanta. 2019 Nov 1;204:337-343. doi: 10.1016/j.talanta.2019.05.100. Epub 2019 Jun 6.

DOI:10.1016/j.talanta.2019.05.100

PMID:31357302

Abstract

Oil red O (ORO) is a commonly used dye for the tissue staining, which recognizes nitroglycerin and cholesterol esters. In this paper, we describe an unexpected finding that ORO can be fluorescently lighted up upon meeting dsDNA with emission peak localized at 600 nm, although it almost does not fluoresce by itself. This property triggered us to test its potential as a dsDNA fluorescence probe. The interaction of dsDNA and ORO was demonstrated by spectral and thermodynamic analysis and several other means. The fluorescence of ORO was gradually increased following the addition of dsDNA, which showed linear response toward dsDNA in the concentration range from 0.02 to 0.64 mg/mL with a detection limit of 0.69 μg/mL. Moreover, ORO showed excellent selectivity when testing with a variety of dsDNA-like biomolecules. Besides, ORO resisted photobleaching and worked very well under biological background. Altogether, we propose here a new nucleic acid lighting up fluorescence probe for double stranded dsDNA detection.

摘要

油红 O（ORO）是一种常用于组织染色的染料，可识别硝化甘油和胆固醇酯。在本文中，我们描述了一个意外的发现，即 ORO 在遇到 dsDNA 时会发出荧光，发射峰位于 600nm 处，尽管它本身几乎不发荧光。这一特性促使我们测试其作为 dsDNA 荧光探针的潜力。通过光谱和热力学分析以及其他几种方法证明了 dsDNA 和 ORO 的相互作用。随着 dsDNA 的加入，ORO 的荧光逐渐增强，在浓度范围从 0.02 到 0.64mg/mL 时对 dsDNA 表现出线性响应，检测限为 0.69μg/mL。此外，在测试各种 dsDNA 样生物分子时，ORO 表现出优异的选择性。此外，ORO 抗光漂白，在生物背景下工作良好。总之，我们在这里提出了一种用于双链 dsDNA 检测的新型核酸点亮荧光探针。