Campen C A, Vale W
Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037.
Endocrinology. 1988 Sep;123(3):1320-8. doi: 10.1210/endo-123-3-1320.
Primary pituitary cell cultures derived from adult male rats were used to explore the interaction between purified 32K ovine inhibin and gonadal steroids on the modulation of basal and GnRH-stimulated secretion of FSH and LH. Purified o-inhibin was a potent inhibitor of basal FSH secretion (IC50 = 3.4 pM) and 10 nM GnRH-stimulated FSH and LH secretion (IC50 = 8.8 and 18.4 pM, respectively). Purified o-inhibin had no effect on basal LH secretion. In this system, the androgens testosterone, 5 alpha-dihydrotestosterone, and androstenedione slightly stimulated basal FSH secretion, and o-inhibin could reverse this stimulatory effect. By contrast, androgens suppress GnRH-stimulated FSH and LH secretion. In combination, androgens and o-inhibin provided greater inhibition of GnRH-mediated gonadotropin secretion than either did alone.
使用从成年雄性大鼠获得的原代垂体细胞培养物来探究纯化的32K羊抑制素与性腺类固醇之间对基础及促性腺激素释放激素（GnRH）刺激的促卵泡激素（FSH）和促黄体生成素（LH）分泌调节的相互作用。纯化的羊抑制素是基础FSH分泌的有效抑制剂（半数抑制浓度[IC50]=3.4皮摩尔）以及10纳摩尔GnRH刺激的FSH和LH分泌的有效抑制剂（IC50分别为8.8和18.4皮摩尔）。纯化的羊抑制素对基础LH分泌无影响。在该系统中，雄激素睾酮、5α-二氢睾酮和雄烯二酮轻微刺激基础FSH分泌，而羊抑制素可逆转这种刺激作用。相比之下，雄激素抑制GnRH刺激的FSH和LH分泌。雄激素和羊抑制素联合使用时，对GnRH介导的促性腺激素分泌的抑制作用比单独使用时更强。
