纯化的绵羊抑制素与类固醇对培养的大鼠垂体细胞促性腺激素释放的相互作用。

Interaction between purified ovine inhibin and steroids on the release of gonadotropins from cultured rat pituitary cells.

作者信息

Campen C A, Vale W

机构信息

Clayton Foundation Laboratories for Peptide Biology, Salk Institute, La Jolla, California 92037.

出版信息

Endocrinology. 1988 Sep;123(3):1320-8. doi: 10.1210/endo-123-3-1320.

DOI:10.1210/endo-123-3-1320

PMID:3136005

Abstract

Primary pituitary cell cultures derived from adult male rats were used to explore the interaction between purified 32K ovine inhibin and gonadal steroids on the modulation of basal and GnRH-stimulated secretion of FSH and LH. Purified o-inhibin was a potent inhibitor of basal FSH secretion (IC50 = 3.4 pM) and 10 nM GnRH-stimulated FSH and LH secretion (IC50 = 8.8 and 18.4 pM, respectively). Purified o-inhibin had no effect on basal LH secretion. In this system, the androgens testosterone, 5 alpha-dihydrotestosterone, and androstenedione slightly stimulated basal FSH secretion, and o-inhibin could reverse this stimulatory effect. By contrast, androgens suppress GnRH-stimulated FSH and LH secretion. In combination, androgens and o-inhibin provided greater inhibition of GnRH-mediated gonadotropin secretion than either did alone.

摘要

使用从成年雄性大鼠获得的原代垂体细胞培养物来探究纯化的32K羊抑制素与性腺类固醇之间对基础及促性腺激素释放激素（GnRH）刺激的促卵泡激素（FSH）和促黄体生成素（LH）分泌调节的相互作用。纯化的羊抑制素是基础FSH分泌的有效抑制剂（半数抑制浓度[IC50]=3.4皮摩尔）以及10纳摩尔GnRH刺激的FSH和LH分泌的有效抑制剂（IC50分别为8.8和18.4皮摩尔）。纯化的羊抑制素对基础LH分泌无影响。在该系统中，雄激素睾酮、5α-二氢睾酮和雄烯二酮轻微刺激基础FSH分泌，而羊抑制素可逆转这种刺激作用。相比之下，雄激素抑制GnRH刺激的FSH和LH分泌。雄激素和羊抑制素联合使用时，对GnRH介导的促性腺激素分泌的抑制作用比单独使用时更强。