Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India.
Antonie Van Leeuwenhoek. 2019 Dec;112(12):1775-1784. doi: 10.1007/s10482-019-01310-7. Epub 2019 Jul 30.
FSH1 belongs to the family of serine hydrolases in yeast and is homologous to the human ovarian tumor suppressor gene (OVAC2). Our preliminary results showed that cells lacking Fsh1p exhibit an increase in cell growth, and a decrease in the expression of AIF1 and NUC1 (apoptosis responsive genes) when compared to the wild type cells. Growth inhibition of cells overexpressing FSH1 is due to induction of cell death associated with cell death markers typical of mammalian apoptosis namely DNA fragmentation, phosphatidylserine externalization, ROS accumulation, Cytochrome c release, and altered mitochondrial membrane potential. When wild type cells were overexpressed with FSH1 there was up regulation of AIF1 level when compared to control cells suggesting that overexpression of FSH1 regulated cell death in yeast.
FSH1 属于酵母丝氨酸水解酶家族，与人类卵巢肿瘤抑制基因（OVAC2）同源。我们的初步结果表明，与野生型细胞相比，缺乏 Fsh1p 的细胞表现出细胞生长增加，以及 AIF1 和 NUC1（细胞凋亡响应基因）的表达减少。过表达 FSH1 的细胞的生长抑制是由于诱导与哺乳动物细胞凋亡典型的细胞死亡标记物相关的细胞死亡，即 DNA 片段化、磷脂酰丝氨酸外翻、ROS 积累、细胞色素 c 释放和线粒体膜电位改变。当野生型细胞过表达 FSH1 时，与对照细胞相比，AIF1 水平上调，这表明 FSH1 的过表达调节了酵母细胞的死亡。
