通过比较慢性重度牙周炎患者和非慢性重度牙周炎患者龈沟液中膜组织延伸刺突蛋白水平来评估其作为牙周病生物标志物的研究——一项初步研究。

Assessment of membrane-organizing extension spike protein as a biomarker for periodontal disease by comparing its level in gingival crevicular fluid in individuals with and without chronic severe periodontitis - A pilot study.

作者信息

Sreedharan Anjali, Shereef Mohammed, Perayil Jayachandran, Fenol Angel, Vyloppillil Rajesh, Balakrishnan Biju

机构信息

Department of Periodontics, Amrita School of Dentistry, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India.

出版信息

J Indian Soc Periodontol. 2019 Jul-Aug;23(4):329-333. doi: 10.4103/jisp.jisp_694_18.

DOI:10.4103/jisp.jisp_694_18

PMID:31367129

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6628777/

Abstract

BACKGROUND

Membrane-organizing extension spike protein (Moesin) is a cytoskeletal protein expressed in the gingival crevicular fluid (GCF) which may play a role in the immune response in periodontal disease. The objective of this study was to evaluate whether Moesin can be used as a biomarker for periodontal disease.

MATERIALS AND METHODS

Thirty patients satisfying the required inclusion criteria were selected from those reporting to the out patient (OP) of the department of periodontics and divided into two groups: Group A - systemically healthy controls with no periodontitis and Group B - systemically healthy controls with chronic severe periodontitis. Periodontal parameters were recorded. GCF was collected, and Moesin levels in the two groups were assessed using enzyme-linked immunosorbent assay. Scaling and root planing (SRP) was done in Group B patients who were reviewed, and samples were collected again after 4 weeks and analyzed.

RESULTS

At baseline, the mean GCF Moesin level in Group A was 666.95 ± 471.872 pg/ml, while in Group B, it was found to be 27435.35 ± 14179.77 pg/ml, which showed a high statistically significant difference on comparison. The mean GCF Moesin level in patients with chronic severe periodontitis was 27435.35 ± 14179.77 pg/ml at baseline, and on review 1 month after SRP, it was found to have undergone a statistically significant reduction to 27161.23 ± 14161.57 pg/ml ( = 0.001).

CONCLUSION

Within the limits of this study, it can be concluded that Moesin can serve as a potential biomarker for periodontal disease.

摘要

背景

膜组织延伸刺突蛋白（埃兹蛋白）是一种在龈沟液（GCF）中表达的细胞骨架蛋白，可能在牙周疾病的免疫反应中发挥作用。本研究的目的是评估埃兹蛋白是否可作为牙周疾病的生物标志物。

材料与方法

从牙周科门诊就诊的患者中选取30例符合纳入标准的患者，分为两组：A组——无牙周炎的全身健康对照组；B组——慢性重度牙周炎的全身健康对照组。记录牙周参数。收集龈沟液，采用酶联免疫吸附测定法评估两组的埃兹蛋白水平。对B组患者进行龈上洁治和根面平整（SRP），复诊时进行复查，并在4周后再次采集样本进行分析。

结果

基线时，A组龈沟液埃兹蛋白平均水平为666.95±471.872 pg/ml，而B组为27435.35±14179.77 pg/ml，比较差异具有高度统计学意义。慢性重度牙周炎患者基线时龈沟液埃兹蛋白平均水平为27435.35±14179.77 pg/ml，SRP后1个月复诊时，发现其显著降低至27161.23±14161.57 pg/ml（P = 0.001）。