利用荧光显微镜对大鼠肝细胞结节内血管构筑进行活体评估。
Intravital assessment of angioarchitecture in rat hepatocellular nodules using fluorescent microscopy.
作者信息
Liu Yi, Lu Tao, Wang Congcong, Li Hui, Xu Ke, Li Peiling
机构信息
Department of Radiology, The First Clinical Hospital of China Medical University, Shenyang 110001, China.
Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
出版信息
Quant Imaging Med Surg. 2019 Jun;9(6):1047-1055. doi: 10.21037/qims.2019.06.11.
BACKGROUND
To prospectively evaluate the stepwise changes that occur in intra-nodular microvessels and microcirculation during the carcinogenesis process of hepatocellular nodules by using fluorescent microscopy, and to compare these with pathological changes.
METHODS
Forty-five 10-week-old male Wistar rats received drinking water containing N-nitrosomorpholine at 10 mg/100 mL for 18weeks to develop multiple hepatocellular carcinomas (HCC) and dysplastic nodules (DN) in the liver; meanwhile, the non-lesion liver tissues become fibrotic. The microvascular morphological change and hemodynamic change of two lesion areas (HCC or DN) and one non-lesion area in each rat were observed with fluorescent microscope. After microscopy, 90 nodules and 45 non-lesion liver tissues that were observed were removed for pathological study. The microvessel density (MVD), branch density (BD), and cell density (CD) of these lesions were compared with the Kruskal-Wallis test and Mann-Whitney test, with an overall statistical significance of 0.05.
RESULTS
The intra-nodular microvessels appeared tortuous, with irregular branching and abrupt diameter changes to form irregular convoluted networks in the HCC. This was distinctly different from the appearance of DN and non-lesion liver parenchyma. The MVD and BD of HCC were less than that of the DN and non-lesion liver parenchyma (P<0.01), and the BD of DN was also less than that of the non-lesion liver parenchyma (P<0.05). However, the MVD of the DN was similar to that of the non-lesion liver parenchyma (P>0.05). The CD of HCC was more than that of the DN and non-lesion liver parenchyma (P<0.05), and the CD of DN was also more than that of the non-lesion liver parenchyma (P<0.05).
CONCLUSIONS
Concurrent with the carcinogenesis process of the hepatocellular nodule, both the intra-nodular microvascular morphology and hemodynamics were stepwise changed, and the number of the intravascular lumen of intranodular microvessels decreased due to the infiltration and compression of intra-nodular parenchymal cells.
背景
通过荧光显微镜前瞻性评估肝细胞结节癌变过程中结节内微血管和微循环的逐步变化，并将其与病理变化进行比较。
方法
45只10周龄雄性Wistar大鼠饮用含10mg/100mL N-亚硝基吗啉的饮用水18周，以在肝脏中形成多个肝细胞癌（HCC）和发育异常结节（DN）；同时，非病变肝脏组织发生纤维化。用荧光显微镜观察每只大鼠两个病变区域（HCC或DN）和一个非病变区域的微血管形态变化和血流动力学变化。显微镜检查后，切除观察到的90个结节和45个非病变肝脏组织进行病理研究。采用Kruskal-Wallis检验和Mann-Whitney检验比较这些病变的微血管密度（MVD）、分支密度（BD）和细胞密度（CD），总体统计学显著性为0.05。
结果
HCC结节内微血管呈扭曲状，分支不规则，直径突然改变，形成不规则的盘绕网络。这与DN和非病变肝实质的外观明显不同。HCC的MVD和BD低于DN和非病变肝实质（P<0.01），DN的BD也低于非病变肝实质（P<0.05）。然而，DN的MVD与非病变肝实质相似（P>0.05）。HCC的CD高于DN和非病变肝实质（P<0.05），DN的CD也高于非病变肝实质（P<0.05）。
结论
在肝细胞结节癌变过程中，结节内微血管形态和血流动力学均发生逐步变化，结节内微血管的血管腔数量因结节内实质细胞的浸润和压迫而减少。
Zotero 插件