Department of Pharmacology, Jinan University, Guangzhou, China.
Department of Physiology, School of Medicine, Jinan University, Guangzhou, China.
Cell Biochem Funct. 2019 Oct;37(7):486-493. doi: 10.1002/cbf.3419. Epub 2019 Aug 1.
Although the spontaneous chloride currents (SCC) have been well studied in the normal cells, its properties and roles in neoplasms cells are still unknown. Here, we found that the SCC was manifested in the poorly differentiated human nasopharyngeal carcinoma CNE-2Z cells, with some differences such as lower occurrence and bigger current density than those of the volume-activated chloride currents (VACC). NPPB, a chloride channel blocker, inhibited the SCC much stronger than the VACC. Down-regulation of chloride channel -3 (ClC-3), a volume and mechanically dependent ion channel, could significantly decrease the VACC, but not in SCC. The occurrence, latency, and mean density of the SCC were much lower in the normal nasopharyngeal NP69-SV40T cells than those in CNE-2Z cells. Our results demonstrated that the spontaneous electrical reactivity of neoplasm cells is higher than that of normal cells, which probably relates to their high physiological activity of neoplasm cells. SIGNIFICANCE OF THE STUDY: Spontaneous chloride currents (SCC) are well known in excitable tissues and regulate a variety of physiological and pathophysiological processes. During our researching on the volume-activated chloride currents (VACC) in human nasopharyngeal carcinoma CNE-2Z cells, SCC could be also observed with different properties from VACC. Meanwhile, the occurrence, latency, and mean density of the SCC were much higher in CNE-2Z cells than those in normal nasopharyngeal NP69-SV40T cells. Our results revealed the expression and characteristics of SCC in carcinoma cells and provided a preliminary experimental basis for further exploring the function of SCC in tumour cell biology.
虽然自发性氯离子电流（SCC）在正常细胞中已经得到了很好的研究，但它在肿瘤细胞中的特性和作用仍不清楚。在这里，我们发现 SCC 表现在低分化的人鼻咽癌 CNE-2Z 细胞中，与体积激活氯离子电流（VACC）相比，其发生频率较低，电流密度较大。氯离子通道阻断剂 NPPB 对 SCC 的抑制作用明显强于 VACC。氯离子通道-3（ClC-3）的下调，一种体积和机械依赖性离子通道，可以显著降低 VACC，但不能降低 SCC。正常鼻咽 NP69-SV40T 细胞中的 SCC 的发生、潜伏期和平均密度均明显低于 CNE-2Z 细胞。我们的结果表明，肿瘤细胞的自发性电反应性高于正常细胞，这可能与其肿瘤细胞的高生理活性有关。研究意义：自发性氯离子电流（SCC）在可兴奋组织中广为人知，调节着各种生理和病理生理过程。在我们对人鼻咽癌 CNE-2Z 细胞的体积激活氯离子电流（VACC）的研究中，也可以观察到与 VACC 不同特性的 SCC。同时，CNE-2Z 细胞中的 SCC 发生、潜伏期和平均密度均明显高于正常鼻咽 NP69-SV40T 细胞。我们的结果揭示了 SCC 在癌细胞中的表达和特征，并为进一步探讨 SCC 在肿瘤细胞生物学中的功能提供了初步的实验基础。
