Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
Third Institute of Oceanography, Ministry of Natural Resources, PR China, Xiamen, 361005, China.
Chemosphere. 2019 Dec;237:124418. doi: 10.1016/j.chemosphere.2019.124418. Epub 2019 Jul 25.
Antibiotic contaminants have become a severe environmental problem in recent years and finding effective ways to deal with this issue is of great importance. In this study, Phanerochaete chrysosporium was used to degrade sulfadiazine (SDZ), which is frequently detected in the culture medium of isolates from soil and surface water systems. The results demonstrate that 10 mg L SDZ can be completely degraded by P. chrysosporium under conditions of pH 5.7 and 30 °C within 6 days. The Q-Exactive-MS/MS analysis identified and confirmed several different SDZ degradation intermediates, and four proposed degradation pathways of SDZ were deduced. Moreover, enzyme activity tests revealed that manganese peroxidase and ligninolytic peroxidase played important roles in SDZ degradation. Moreover, a transcriptome analysis method was performed to explore the mechanism and pathways of SDZ degradation by P. chrysosporium in greater detail. The results of GO and KEGG analysis strongly suggest that the metabolism pathway is significantly activated and plays an important role in antibiotic degradation. Further, this is the first study to identify SDZ degradation intermediates and two main intermediates were found to be involved in possible SDZ degradation pathways. This study is also the first report results from RNA sequencing to evaluate genome-wide changes of P. chrysosporium to further explore SDZ degradation mechanism.
近年来,抗生素污染物已成为一个严重的环境问题,寻找有效方法来应对这一问题至关重要。在本研究中,使用黄孢原毛平革菌(Phanerochaete chrysosporium)来降解磺胺嘧啶(SDZ),SDZ 经常在土壤和地表水系统分离物的培养基中被检测到。结果表明,在 pH 值为 5.7 和 30°C 的条件下,10mg/L 的 SDZ 在 6 天内可被 P. chrysosporium 完全降解。Q-Exactive-MS/MS 分析鉴定并确认了几种不同的 SDZ 降解中间产物,并推导出了 4 条 SDZ 降解途径。此外,酶活性测试表明锰过氧化物酶和木质素过氧化物酶在 SDZ 降解中发挥重要作用。此外,还采用转录组分析方法更详细地研究了 P. chrysosporium 降解 SDZ 的机制和途径。GO 和 KEGG 分析的结果强烈表明代谢途径明显被激活,并在抗生素降解中发挥重要作用。此外,这是首次鉴定 SDZ 降解中间产物的研究,发现两种主要中间产物可能参与 SDZ 降解途径。本研究也是首次利用 RNA 测序来评估 P. chrysosporium 的全基因组变化,以进一步探索 SDZ 降解机制。