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黄曲霉孢子表面蛋白质组以及疏水蛋白A/RodA蛋白变体的数据集。

Dataset for the spore surface proteome and hydrophobin A/RodA proteoforms of A.flavus.

作者信息

Shait Mohammed Mohammed Razeeth, Balamurgan Muthu Kumar, Amrathlal Rabbind Singh, Kannan Priyadharshini, Jayapal Jeya Maheshwari, Namperumalsamy Venkatesh Prajna, Prajna Lalitha, Dharmalingam Kuppamuthu

机构信息

Department of Proteomics, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, TamilNadu, India.

Department of Microbiology, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, TamilNadu, India.

出版信息

Data Brief. 2019 Mar 15;23:103817. doi: 10.1016/j.dib.2019.103817. eCollection 2019 Apr.

Abstract

Fungal keratitis is a major sight-threatening corneal infection: and mycotic keratitis is more common in tropical parts of the world including India. and are the predominant causative agents of corneal infection. We extracted conidial surface proteins of from saprophyte and clinical isolates and analyzed the proteins using high resolution mass spectrometry. The data revealed ecotype specific alteration in surface proteome since the proteome profile of the clinical isolates and saprophyte showed significant differences. Detailed examination of the mass spec data of RodA proteins extracted from polyacrylamide gels revealed the presence of two proteoforms of this protein. We also identified the mechanism of formation of these two isoforms. Detailed analysis of this data and the conclusions derived are described in the article, "Identification of the proteoforms of surface localized Rod A of and determination of the mechanism of proteoform generation" [1].

摘要

真菌性角膜炎是一种严重威胁视力的角膜感染

霉菌性角膜炎在包括印度在内的世界热带地区更为常见。 和 是角膜感染的主要致病因素。我们从腐生菌和临床分离株中提取了 的分生孢子表面蛋白,并使用高分辨率质谱对这些蛋白进行了分析。数据显示,由于临床分离株和腐生菌的蛋白质组谱存在显著差异,表面蛋白质组存在生态型特异性改变。对从聚丙烯酰胺凝胶中提取的RodA蛋白的质谱数据进行详细检查后发现,该蛋白存在两种蛋白形式。我们还确定了这两种异构体的形成机制。本文“ 和 表面定位的Rod A蛋白形式的鉴定及蛋白形式产生机制的确定”[1]中描述了对这些数据的详细分析以及得出的结论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e363/6660596/a931b49f9d2f/gr1.jpg

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