Hata Akihisa, Hasegawa Momoko, Yamauchi Takenori, Otomo Yuki, Miura Motofumi, Yamanaka Kenzo, Yamano Yuko, Fujitani Noboru, Endo Ginji
Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoino-oka, Imabari, Ehime, 794-8555, Japan.
Department of Medical Risk Management, Graduate School of Risk and Crisis Management, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan.
Heliyon. 2019 Jul 19;5(7):e02079. doi: 10.1016/j.heliyon.2019.e02079. eCollection 2019 Jul.
Seaweeds contain large amounts of organoarsenic compounds, mostly arsenosugars (AsSug) and arsenolipids (AsLipid). AsSug is mainly metabolized into dimethylarsinic acid (DMA ) in humans. However, this metabolic process is not well understood. We investigated the metabolism of an AsSug, 3-[5'-deoxy-5'-(dimethylarsinoyl)-β-ribofuranosyloxy]-2-hydroxypropylene glycol (AsSug328), in the gastrointestinal tract using an artificial gastrointestinal digestion system. AsSug328 was incubated with gastric juice for 4 h, with bile-pancreatic juice for 0.5 h, and finally with enteric bacteria solution for 24 h. The conversion of arsenic compounds after artificial digestion was analyzed by HPLC-ICP-MS and HPLC-ESI-Q-TOF-MS. Our results show that artificial gastrointestinal digestion converted AsSug328 into thio-AsSug328. However, no formation of DMA was detected. Under the artificial digestion system, the 5-deoxyribofuranose structure of AsSug was maintained. Therefore, AsSug should be absorbed in the intestinal tract after its sugar moiety is partially decomposed. They are then possibly metabolized to DMA in the liver and subsequently excreted through urine.
海藻含有大量有机砷化合物，主要是砷糖（AsSug）和砷脂（AsLipid）。砷糖在人体内主要代谢为二甲基胂酸（DMA）。然而，这一代谢过程尚未完全明确。我们使用人工胃肠消化系统研究了一种砷糖，即3-[5'-脱氧-5'-(二甲基胂酰基)-β-呋喃核糖氧基]-2-羟基丙二醇（AsSug328）在胃肠道中的代谢情况。将AsSug328与胃液孵育4小时，与胆胰液孵育0.5小时，最后与肠道细菌溶液孵育24小时。通过高效液相色谱-电感耦合等离子体质谱（HPLC-ICP-MS）和高效液相色谱-电喷雾-四极杆-飞行时间质谱（HPLC-ESI-Q-TOF-MS）分析人工消化后砷化合物的转化情况。我们的结果表明，人工胃肠消化将AsSug328转化为硫代-AsSug328。然而，未检测到DMA的形成。在人工消化系统下，砷糖的5-脱氧呋喃核糖结构得以保留。因此，砷糖在其糖部分被部分分解后应在肠道中被吸收。然后它们可能在肝脏中代谢为DMA，随后通过尿液排出。
