含豆芽甲醇提取物的双功能化纳米脂质体的制备、开发与评价：作为大豆油氧化稳定性的有效天然抗氧化剂
Formulation, development and evaluation of bifunctionalized nanoliposomes containing sprout methanolic extract: as effective natural antioxidants on the oxidative stability of soybean oil.
作者信息
Sayyed-Alangi S Zahra, Nematzadeh Meysam
机构信息
Department of Chemistry, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
Department of Food Engineering, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
出版信息
BMC Chem. 2019 Jun 28;13(1):77. doi: 10.1186/s13065-019-0594-7. eCollection 2019 Dec.
BACKGROUND
The various extracts of ( clover) sprout was obtained by using different solvents and microwave assisted extraction in the present study. Then, the bifunctionalized nanoliposomes were prepared and added to soybean oil for evaluating their effect on deferring the oxidation process.
METHODS
The total phenol and antioxidant activity of the extracts was determined by using the free radical scavenging assay. Then, various nanoliposomal structures of the methanolic extract of clover sprout (PCSE) were prepared by using six several formulations containing different ratios of soybean oil, lecithin and the extract. Afterward, the most stable nanoliposome was bifunctionalized by using WPC and pectin (PCSEN-W and PCSEN-WP, respectively). The size and zeta potential of nanoparticles were measured. Furthermore, in order to evaluate the effects of PCSE, PCSEN, PCSEN-W and PCSEN-WP at 100-300 ppm concentrations in deferring the oxidation process of soybean oil, the heat treatment tests were applied (PV and TBA) at 63 °C within a 20-day period.
RESULTS
The methanolic extract had the highest level of total phenol and antioxidant activity. The results of creaming index and microencapsulation efficiency were exhibited that formulation containing 30% oil, 5% lecithin and 2% the extract was led to the production of the most stable nanoliposomal structure (PCSEN). The size of nanoparticles was in the range of 282.5-491.2 nm. Zeta potential of the samples was obtained in the range between - 56.9 and - 36.3 mV. Polydispersity index of them was ranged from 0.424 to 0.541. The results were confirmed the existence of stable nanoliposomal systems. The results of the PV and TBA values of the extracts in free and nanoliposomal forms were shown that the nanoliposomal forms had very good antioxidant activity against the oxidation process in soybean oil.
背景
本研究采用不同溶剂及微波辅助萃取法获得了三叶草芽的各种提取物。然后，制备了双功能化纳米脂质体并添加到大豆油中，以评估其对延缓氧化过程的作用。
方法
采用自由基清除法测定提取物的总酚含量和抗氧化活性。然后，使用六种含有不同比例大豆油、卵磷脂和提取物的配方，制备了三叶草芽甲醇提取物（PCSE）的各种纳米脂质体结构。之后，分别用乳清蛋白浓缩物（WPC）和果胶对最稳定的纳米脂质体进行双功能化（分别为PCSEN-W和PCSEN-WP）。测量了纳米颗粒的尺寸和zeta电位。此外，为了评估浓度为100 - 300 ppm的PCSE、PCSEN、PCSEN-W和PCSEN-WP在延缓大豆油氧化过程中的作用，在63℃下进行了为期20天的热处理试验（过氧化值和硫代巴比妥酸值）。
结果
甲醇提取物的总酚含量和抗氧化活性最高。乳析指数和微囊化效率的结果表明，含有30%油、5%卵磷脂和2%提取物的配方可产生最稳定的纳米脂质体结构（PCSEN）。纳米颗粒的尺寸在282.5 - 491.2 nm范围内。样品的zeta电位在-56.9至-36.3 mV之间。其多分散指数在0.424至0.541之间。结果证实了稳定纳米脂质体系统的存在。游离形式和纳米脂质体形式提取物的过氧化值和硫代巴比妥酸值结果表明，纳米脂质体形式对大豆油的氧化过程具有非常好的抗氧化活性。
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