Department of Emergency, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province 450052, China.
National Institute For Viral Disease Control and Prevention, Chinese Center For Disease Control and Prevention, Beijing City, 102206, China.
Life Sci. 2019 Sep 15;233:116728. doi: 10.1016/j.lfs.2019.116728. Epub 2019 Aug 3.
Impaired wound healing is a serious concern of uncontrolled hyperglycemia that can lead to gangrene, and even death. There is an urgent need to look for better alternative therapy because of the undesirable side effects of currently available synthetic drugs in the market. Syringic acid (SA) is a natural phenolic compound abundantly available in edible fruits and plants. In this study, wound healing activities of 2.5% and 5.0% SA were evaluated in type 2 diabetic rats using incisional wound model. SA-treated diabetic wounds showed faster rate of wound closure and epithelization with enhanced contents of hydroxyproline and protein compared to diabetic wounds. SA effectively prevents alterations in blood glucose levels, serum insulin and dyslipidemia in diabetic wound rats. The SA-treated diabetic wounds after 14 days of treatment demonstrated inhibition of pro-inflammatory response (NF-κB p65, TNF-α, IL-1β, IL-8 and IL-2) with improvement in anti-inflammatory response (IL-10), inhibited the elevated oxidative stress and decreased the concentrations of matrix metalloproteinases (MMP-2, -8 and -9) and increased the concentrations of TIMP-1 & TIMP- 2. Furthermore, the diabetic wounds were presented with an increase in expression of CD 31 and 68, growth factors (TGF-β1, collagen-I and α-SMA and VEGF) with significant improvement in collagen deposition, re-epithelialization and complete skin structure as revealed by histological analysis after treatment of diabetic wounds with SA for 14 days. Hence, the results of this study designate that SA significantly improves wound healing in diabetic rats and could be used as a potential therapy for treatment of diabetic wounds.
创面愈合受损是血糖失控的严重问题，可能导致坏疽，甚至死亡。由于目前市场上合成药物存在不良副作用，因此迫切需要寻找更好的替代疗法。丁香酸（SA）是一种天然酚类化合物，在可食用的水果和植物中大量存在。在这项研究中，通过切口创面模型评估了 2.5%和 5.0%SA 对 2 型糖尿病大鼠创面愈合的作用。与糖尿病创面相比，SA 处理的糖尿病创面的愈合和上皮化速度更快，羟脯氨酸和蛋白质含量更高。SA 能有效防止糖尿病创面大鼠血糖、血清胰岛素和血脂异常的改变。经过 14 天治疗后，SA 处理的糖尿病创面表现出抑制促炎反应（NF-κB p65、TNF-α、IL-1β、IL-8 和 IL-2），并改善抗炎反应（IL-10），抑制氧化应激升高，降低基质金属蛋白酶（MMP-2、-8 和 -9）浓度，增加 TIMP-1 和 TIMP-2 浓度。此外，糖尿病创面的 CD31 和 68、生长因子（TGF-β1、I 型胶原和 α-SMA 和 VEGF）的表达增加，胶原沉积、再上皮化和完整皮肤结构得到显著改善，这是在糖尿病创面用 SA 治疗 14 天后通过组织学分析观察到的。因此，这项研究的结果表明，SA 能显著改善糖尿病大鼠的创面愈合，可作为治疗糖尿病创面的潜在疗法。
