MCF7乳腺癌细胞系的蛋白质组学分析
Proteomic Analysis of the MCF7 Breast Cancer Cell Line.
作者信息
Hardouin Julie, Canelle Ludovic, Vlieghe Céline, Lasserre Jean-Paul, Caron Michel, Joubert-Caron Raymonde
机构信息
Laboratory of Protein Biochemistry and Proteomics, UMR CNRS 7033 (BioMoCeTi), UFR SMBH, Paris13 University, 93017 Bobigny Cedex, France.
Laboratory of Protein Biochemistry and Proteomics, UMR CNRS 7033 (BioMoCeTi), UFR SMBH, Paris13 University, 93017 Bobigny Cedex, France
出版信息
Cancer Genomics Proteomics. 2006 Nov-Dec;3(6):355-368. Epub 2006 Nov 1.
BACKGROUND
The MCF7 breast cancer cell line is a cellular model for breast cancer studies and marker discovery. Therefore, a better knowledge of its proteome is a prerequisite for a more efficient use of this model.
MATERIALS AND METHODS
Proteins expressed during the exponential growth phase of MCF7 cells were analyzed and mapped using two-dimensional gel electrophoresis and mass spectrometry.
RESULTS
From the spots excised from preparative gels of whole-cell extracts, a subset of 368 different polypeptides, corresponding to 249 different proteins, was identified. These polypeptides were positioned on a silver-stained gel to construct a reference map.
CONCLUSION
The data allowed the construction of the most extensive reference map for MCF7 published to date, with 189 novel proteins, which had not been previously listed on maps, and are now accessible on World 2D-PAGE database, providing a basis for further studies on MCF7.
背景
MCF7乳腺癌细胞系是用于乳腺癌研究和标志物发现的细胞模型。因此，更好地了解其蛋白质组是更有效地利用该模型的前提条件。
材料与方法
使用二维凝胶电泳和质谱分析并绘制MCF7细胞指数生长期表达的蛋白质。
结果
从全细胞提取物制备凝胶上切下的斑点中，鉴定出了368种不同多肽的一个子集，对应于249种不同蛋白质。这些多肽定位在银染凝胶上以构建参考图谱。
结论
这些数据构建了迄今为止发布的最全面的MCF7参考图谱，其中有189种新蛋白质，以前未列在图谱上，现在可在世界二维电泳数据库中获取，为进一步研究MCF7提供了基础。
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