At Oeste Paulista University, Presidente Prudente, in São Paulo, Brazil, Amanda Feba Tetila, MSc, is a physiotherapist and postgraduate student; Maria Rosa Santos Breda, MSc, is a veterinarian and postgraduate student; Rosa Maria Barilli Nogueira, PhD, is a Professor, Postgraduate Program in Animal Science; Gisele Alborghetti Nai, PhD, is a Professor, Postgraduate Program in Animal Science; and Cecília Braga Laposy, PhD, is a Professor, Postgraduate Program in Animal Science. Acknowledgment: Support for this article was provided by the Foundation for Research Support of the State of São Paulo (FAPESP, process no. 2016/25938-0). The authors have disclosed no other financial relationships related to this article. Submitted September 3, 2018; accepted in revised form November 26, 2018; published online ahead of print August 9, 2019.
Adv Skin Wound Care. 2019 Sep;32(9):1-5. doi: 10.1097/01.ASW.0000577136.88748.68.
To test the hypothesis that platelet-rich plasma (PRP) improves wound healing in rabbits by analyzing morphologic alterations in lesions with the use of PRP alone or combined with rosuvastatin (RSV).
Eight adult male New Zealand rabbits were used in this study. Researchers created four wounds on the back of each rabbit with an 8-mm punch. The control wound was treated with a 0.9% sodium chloride solution. Experimental wounds were treated with PRP, RSV, or both PRP and RSV. Dressings were changed with a new application of PRP and RSV every 4 days for 16 days. Wounds were biopsied on days 0, 7, 10, 14, and 17 for histopathologic evaluation of the scar tissue.
Histopathology revealed reepithelialization in 100% of wounds treated with PRP alone after 17 days of treatment, compared with 50% of wounds treated with RSV alone and 75% of the wounds treated with PRP and RSV. Further, combining RSV and PRP reduced blood loss. The use of PRP alone induced 100% neovascularization, compared with 50% and 62.5% in wounds treated with PRP and RSV and RSV alone, respectively. All experimental wounds had a higher percentage of collagen fibers on day 17 postlesion when compared with control wounds (78.27% ± 4.69%). There were no significant differences among treatments; however, wounds treated with RSV alone had the lowest amount of collagen fibers (85.98% ± 3.51%). Wounds treated with PRP alone or PRP and RSV had 90.07% ± 6.20% and 90.76% ± 3.51% collagen fibers, respectively.
The results of this study indicate that PRP elicits higher healing activity in the first 7 days postlesion. Treatments with RSV alone or RSV and PRP did enhance other healing phases.
通过分析单独使用富血小板血浆（PRP）或联合使用瑞舒伐他汀（RSV）治疗病变后的形态学变化，验证 PRP 可改善兔子伤口愈合的假设。
本研究使用了 8 只成年雄性新西兰兔。研究人员用 8mm 的打孔器在每只兔子的背部制造了 4 个伤口。对照组的伤口用 0.9%氯化钠溶液处理。实验组的伤口分别用 PRP、RSV 或 PRP 和 RSV 联合处理。每隔 4 天用新的 PRP 和 RSV 更换敷料，共 16 天。在第 0、7、10、14 和 17 天对伤口进行活检，以评估疤痕组织的组织病理学。
组织病理学显示，单独使用 PRP 治疗的 17 天后，所有伤口均完全上皮化，而单独使用 RSV 治疗的伤口有 50%上皮化，联合使用 PRP 和 RSV 治疗的伤口有 75%上皮化。此外，联合使用 RSV 和 PRP 可减少出血。单独使用 PRP 诱导了 100%的新生血管形成，而联合使用 PRP 和 RSV 以及单独使用 RSV 的新生血管形成比例分别为 50%和 62.5%。与对照组相比，所有实验组在伤后第 17 天的胶原纤维百分比均较高（78.27%±4.69%）。但各组间无显著差异；然而，单独使用 RSV 治疗的伤口胶原纤维含量最低（85.98%±3.51%）。单独使用 PRP 或 PRP 和 RSV 治疗的伤口胶原纤维含量分别为 90.07%±6.20%和 90.76%±3.51%。
本研究结果表明，PRP 在伤后前 7 天可引发更高的愈合活性。单独使用 RSV 或 RSV 和 PRP 治疗均可增强其他愈合阶段。
