基因组挖掘与嘌呤环酰胺的酶法全生物合成
Genome Mining and Enzymatic Total Biosynthesis of Purincyclamide.
机构信息
State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, School of Life Sciences, Nanjing University, Nanjing 210023, China.
State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
出版信息
Org Lett. 2019 Sep 6;21(17):6825-6829. doi: 10.1021/acs.orglett.9b02461. Epub 2019 Aug 13.
A cyclodipeptide synthase-containing gene cluster () was identified in NA4264 through genome mining. Heterologous expression of the cryptic gene cluster in led to the production of a new highly modified cyclodipeptide named purincyclamide (). Bioinformatic analysis and gene knockout experiments revealed the biosynthetic pathway. The production of was achieved through a one-pot enzymatic reaction.
通过基因组挖掘，在 NA4264 中鉴定出一个含有环二肽合酶的基因簇（）。在 中异源表达该隐性基因簇导致产生了一种新的高度修饰的环二肽，命名为 purincyclamide（）。生物信息学分析和基因敲除实验揭示了其生物合成途径。通过一锅酶反应实现了 的生产。
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