Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Microb Drug Resist. 2020 Jan;26(1):46-53. doi: 10.1089/mdr.2018.0469. Epub 2019 Aug 16.
The aim of this study was to evaluate the resistance and virulence characteristics of strains of clinical and environmental origins over a 5-year period in Iran and to determine the genetic diversity of strains using pulsed-field gel electrophoresis (PFGE) method. A total of 20 strains were collected from 850 stool samples of patients with diarrhea, and 18 e spp. including 10 were collected from water, food, and vegetable samples. The most frequently isolated strains belonged to biotype (BT) 1A (83.33%). No BT4 was detected that can be attributed to the absence of pig animal reservoir in Iranian food chain. The most frequent chromosomal virulence genes among the isolates were (100%), tA (67%), ystB (83%), (20%), and (17%). The most frequent chromosomal virulence genes among non- spp. isolates were (87.5%), (37.5%), and (37.5%). None of the isolates harbored plasmid origin virulence genes. None of the isolates was resistant to ciprofloxacin, gentamicin, tetracycline, cotrimoxazole, and chloramphenicol, whereas 90% of the and 62.5% of the spp. strains were resistant to ampicillin. PFGE genotyping showed a heterogeneous population of highly susceptible spp. in both clinical and environmental samples, putting forward a good prognosis in the treatment of patients with yersiniosis. The occurrence of biotype 1A with genotype in clinical strains implies the significance of , , and gene products in turning of naturally nonpathogenic biotype 1A strains into clinically important pathogens.
本研究旨在评估 5 年间伊朗临床和环境来源的 株的耐药性和毒力特征，并使用脉冲场凝胶电泳（PFGE）方法确定 株的遗传多样性。从 850 份腹泻患者粪便样本中采集了 20 株 株，从水、食物和蔬菜样本中采集了 18 株 包括 10 株 株。分离出的 株中最常见的生物型（BT）为 1A（83.33%）。未检测到 BT4，这归因于伊朗食物链中没有猪动物宿主。在分离出的 株中最常见的染色体毒力基因是 （100%）、tA（67%）、ystB（83%）、 （20%）和 （17%）。非 株分离株中最常见的染色体毒力基因是 （87.5%）、 （37.5%）和 （37.5%）。没有分离株携带质粒来源的毒力基因。没有分离株对环丙沙星、庆大霉素、四环素、复方新诺明和氯霉素耐药，而 株和 株的 90%和 62.5%对氨苄西林耐药。PFGE 基因分型显示临床和环境样本中高度敏感的 株呈异质群体，这对治疗耶尔森菌病患者提出了良好的预后。临床株中 1A 生物型与 基因型的出现表明 、 、 和 基因产物在将天然非致病性 1A 生物型菌株转化为临床重要病原体方面的重要性。
