DNA 碱基的反应性修饰用于标记、生物缀合和交联。
Reactive modifications of DNA nucleobases for labelling, bioconjugations, and cross-linking.
机构信息
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610 Prague 6, Czech Republic; Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic.
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610 Prague 6, Czech Republic; Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic.
出版信息
Curr Opin Chem Biol. 2019 Oct;52:136-144. doi: 10.1016/j.cbpa.2019.07.007. Epub 2019 Aug 12.
Modification of DNA with reactive groups and their post-synthetic transformations are useful for labelling, imaging, bioconjugations and cross-linking with other (bio)molecules. This review summarizes the recent progress in this field and covers transformations of oxo groups, cycloadditions, conjugate additions, alkylations, cross-couplings and other reactions. Examples of applications are given and the practicability and scope of the reactions are discussed.
用反应基团修饰 DNA 及其后续的合成转化对于标记、成像、生物偶联和与其他（生物）分子交联非常有用。本综述总结了该领域的最新进展，涵盖了氧化基团的转化、环加成、共轭加成、烷基化、交叉偶联和其他反应。给出了应用实例，并讨论了反应的实用性和范围。
Zotero 插件