家蚕丝腺中微小RNA的基因操作
Genetic Manipulation of MicroRNAs in the Silk Gland of Silkworm, .
作者信息
Wang Wei, Wang Xinran, Li Xuemei, Pu Qian, Luo Chengyi, Xu Lili, Peng Xinyue, Liu Shiping
机构信息
1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
2Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing, 400715 People's Republic of China.
出版信息
Biol Proced Online. 2019 Aug 15;21:16. doi: 10.1186/s12575-019-0102-4. eCollection 2019.
BACKGROUND
MicroRNAs (miRNAs) are a class of non-coding RNAs with important post-transcriptional regulatory functions. To reveal the function of miRNAs , the critical step is to change their expression levels in the tissues or organs. In this work, we explored the application of several important genetic techniques in altering the expression of silk gland-specific miR-274 of silkworm ().
RESULTS
Injection of synthesized microRNA mimics and antagomirs exerted no effect on the expression of miR-274 in the silk gland, miR-274 sponge specifically absorbed miR-274 and down-regulated its expression, transgenic overexpression of miR-274 precursor significantly up-regulated miR-274, and finally tissue-specific CRISPR/Cas9 system achieved deletion of miR-274.
CONCLUSIONS
A practical technical system was established for studying the functions of miRNAs in silk gland of . Our research provides methodological support for the functional study of miRNAs and other noncoding RNAs in the silk gland and more organs in other species.
背景
微小RNA（miRNA）是一类具有重要转录后调控功能的非编码RNA。为揭示miRNA的功能，关键步骤是改变其在组织或器官中的表达水平。在本研究中，我们探索了几种重要的基因技术在改变家蚕丝腺特异性miR - 274表达中的应用。
结果
注射合成的微小RNA模拟物和拮抗剂对丝腺中miR - 274的表达没有影响，miR - 274海绵特异性吸附miR - 274并下调其表达，miR - 274前体的转基因过表达显著上调miR - 274，最终组织特异性CRISPR/Cas9系统实现了miR - 274的缺失。
结论
建立了一个用于研究家蚕丝腺中miRNA功能的实用技术体系。我们的研究为家蚕丝腺及其他物种更多器官中miRNA和其他非编码RNA的功能研究提供了方法学支持。
Zotero 插件