基于生物质 C 点和壳聚糖@AuNPs 与透明质酸的双读数纳米传感器用于测定透明质酸酶。
A dual-readout nanosensor based on biomass-based C-dots and chitosan@AuNPs with hyaluronic acid for determination of hyaluronidase.
机构信息
College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an, People' Republic of China.
Nanshan District Key Lab for Biopolymers and Safety Evaluation, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, People' Republic of China.
出版信息
Luminescence. 2020 Feb;35(1):43-51. doi: 10.1002/bio.3699. Epub 2019 Aug 20.
A dual-signal strategy is proposed based on fluorescent biomass-based carbon dots (BC-dots) and chitosan stabilized AuNPs (CS@AuNPs) to determine hyaluronidase (HAase). BC-dots can induce aggregation of CS@AuNPs nanoparticles with a colour change from red to blue. Positively charged CS@AuNPs interacted with the negatively charged hyaluronic acid (HA) through electrostatic adsorption, and CS@AuNPs maintained stability due to the semirigid coil conformation of HA. However, in the presence of HAase, due to enzymatic hydrolysis of HA by HAase, the CS@AuNPs agglomerated. Based on the change of fluorescence and colour, quantitative analysis of HAase was achieved. Linear ranges for the fluorometric and colorimetric determinations were 2.0-70 U mL and 8-60 U mL , respectively, with a detection limit of 0.27 U mL . This dual-signal sensing system possesses high potential for determination of HAase in biological matrices.
提出了一种基于荧光生物质碳点（BC-dots）和壳聚糖稳定的金纳米粒子（CS@AuNPs）的双信号策略来测定透明质酸酶（HAase）。BC-dots 可以诱导 CS@AuNPs 纳米粒子的聚集，从而使颜色从红色变为蓝色。带正电荷的 CS@AuNPs 通过静电吸附与带负电荷的透明质酸（HA）相互作用，并且由于 HA 的半刚性螺旋构象，CS@AuNPs 保持稳定。然而，在存在 HAase 的情况下，由于 HAase 对 HA 的酶解作用，CS@AuNPs 聚集。基于荧光和颜色的变化，实现了对 HAase 的定量分析。荧光和比色测定的线性范围分别为 2.0-70 U mL 和 8-60 U mL ，检测限为 0.27 U mL 。这种双信号传感系统在生物基质中测定 HAase 具有很大的潜力。
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