Automated Quantitative Analysis of Mitochondrial Morphology.

Mitochondria are dynamic organelles that in most cells behave as a dynamic network and can change their biogenesis and structure depending on the cell needs or as a response to different conditions. Analyzing the architecture of mitochondria is determinant to describe their state and function. In this chapter, image processing techniques are applied in a workflow manner to segment the mitochondrial network and extract the most relevant parameters that enable an accurate morphology analysis. This workflow is programmed with ImageJ macro language and can be applied to automatically analyze multiple cells from multiple images or tiles. When combined with multiwell plates and automated microscopy, this method may allow to perform high content image analysis of hundreds of cells under different conditions.