Bandyopadhyay Amal Kumar, Islam Rifat Nawaz U, Mitra Debanjan, Banerjee Sahini, Goswami Arunava
Department of Biotechnology,The University of Burdwan,Burdwan, West Bengal,India.
Department of Zoology,The University of Burdwan,Burdwan,West Bengal,India.
Bioinformation. 2019 Feb 28;15(2):79-89. doi: 10.6026/97320630015079. eCollection 2019.
We analyzed the water-ferredoxin interaction in mesophilic (moderate temperature) algae (PDB ID: 1AWD) and halophilic (salt-tolerant) archaea (PDB ID: 1DOI) using POWAIND version 2.0 (a protein-water interactions calculation program). It is found that the shell water (SW) is 2.5 fold greater in halophilic ferredoxin than mesophilic ferredoxin. Water-ferredoxin interactions in the core and cavity are the signature of stability. The normalized frequency of such interactions is less in halophilic relative to mesophilic ferredoxin and the halophilic signature for stability by such interactions is negligible. However, the surface dominated with such interactions seems to be important for ferredoxin and oxido-reductase recognition.
我们使用POWAIND 2.0版（一种蛋白质-水相互作用计算程序）分析了嗜温（中温）藻类（蛋白质数据银行ID：1AWD）和嗜盐（耐盐）古菌（蛋白质数据银行ID：1DOI）中的水-铁氧化还原蛋白相互作用。结果发现，嗜盐铁氧化还原蛋白中的壳层水（SW）比嗜温铁氧化还原蛋白中的多2.5倍。核心和腔中的水-铁氧化还原蛋白相互作用是稳定性的标志。相对于嗜温铁氧化还原蛋白，嗜盐铁氧化还原蛋白中此类相互作用的归一化频率较低，且此类相互作用对稳定性的嗜盐特征可忽略不计。然而，以这种相互作用为主的表面似乎对铁氧化还原蛋白和氧化还原酶识别很重要。
