培养细胞中脑癌的多模态定量成像
Multimodal quantitative imaging of brain cancer in cultured cells.
作者信息
Feng Xin, Muzikansky Alona, Ross Alonzo H, Hamblin Michael R, Jermain Peter R, Yaroslavsky Anna N
机构信息
Advanced Biophotonics Laboratory, UMASS Lowell, Lowell, MA 01851, USA.
Massachusetts General Hospital Biostatistics Center, Boston, MA 02114, USA.
出版信息
Biomed Opt Express. 2019 Jul 29;10(8):4237-4248. doi: 10.1364/BOE.10.004237. eCollection 2019 Aug 1.
Abstract
Fluorescence emission, polarization and subcellular localization of methylene blue (MB) were studied in four cancerous and two normal human brain cell lines. Fluorescence emission and polarization images were acquired and analyzed. The co-localization of MB with mitochondria, lysosomes and nuclei of the cells was evaluated. Glioblastoma cells exhibited significantly higher MB fluorescence polarization compared to normal astrocytes. Preferential accumulation of MB in mitochondria of glioblastoma cells may explain higher fluorescence polarization values in cancer cells as compared to normal. These findings may lead to the development of a quantitative method for the detection of brain cancer in single cells.
摘要
在四种癌细胞系和两种正常人类脑细胞系中研究了亚甲蓝（MB）的荧光发射、偏振和亚细胞定位。采集并分析了荧光发射和偏振图像。评估了MB与细胞线粒体、溶酶体和细胞核的共定位情况。与正常星形胶质细胞相比，胶质母细胞瘤细胞表现出显著更高的MB荧光偏振。胶质母细胞瘤细胞线粒体中MB的优先积累可能解释了与正常细胞相比癌细胞中更高的荧光偏振值。这些发现可能会促成一种用于检测单细胞中脑癌的定量方法的开发。
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2
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Front Oncol. 2018 Jul 27;8:280. doi: 10.3389/fonc.2018.00280. eCollection 2018.
3
Discrimination of malignant and normal kidney tissue with short wave infrared dispersive Raman spectroscopy.应用短波长红外分散拉曼光谱法鉴别良恶性肾组织。
J Biophotonics. 2018 Jun;11(6):e201700188. doi: 10.1002/jbio.201700188. Epub 2018 May 16.
4
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Int J Biol Markers. 2018 Jan;33(1):22-32. doi: 10.5301/ijbm.5000301.
5
Recent progress in surface-enhanced Raman spectroscopy for biological and biomedical applications: from cells to clinics.近年来，表面增强拉曼光谱在生物和生物医学应用方面的进展：从细胞到临床。
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