Guzmán Jesús, Castillo Denis, Ojeda Manuel, Sauvain Michel
Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú.
Clínica Médica Cayetano Heredia. Lima, Perú.
Rev Peru Med Exp Salud Publica. 2019 Apr-Jun;36(2):270-274. doi: 10.17843/rpmesp.2019.362.3901. Epub 2019 Aug 22.
In order to evaluate antimicrobial susceptibility and detect specific mutations in the 23S rRNA gene in Helicobacter pylori strains, a cross-sectional study was performed on 95 patients with dyspepsia treated in a private clinic in Lima. Antrum biopsies were collected by endoscopy for isolation and evaluation of antimicrobial susceptibility using the broth microdilution method. The detection of specific mutations was developed by PCR-RFLP. The percentage of infection by Helicobacter pylori was 46.3%. Resistance values of 52.3% to clarithromycin, 29.6% to metronidazole, 45.5% to levofloxacin, and 4.6% to amoxicillin were observed. The percentage of specific A2142G and A2143G mutations associated with clarithromycin resistance was 43.5%. In conclusion, we found that antimicrobial resistance rates and the percentage of Helicobacter pylori strains circulating in a private clinic in Lima were high.
为了评估幽门螺杆菌菌株的抗菌药敏性并检测23S rRNA基因中的特定突变，对在利马一家私人诊所接受治疗的95例消化不良患者进行了一项横断面研究。通过内窥镜检查采集胃窦活检组织，采用肉汤微量稀释法进行分离和抗菌药敏性评估。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测特定突变。幽门螺杆菌感染率为46.3%。观察到对克拉霉素的耐药率为52.3%，对甲硝唑的耐药率为29.6%，对左氧氟沙星的耐药率为45.5%，对阿莫西林的耐药率为4.6%。与克拉霉素耐药相关的特定A2142G和A2143G突变的百分比为43.5%。总之，我们发现利马一家私人诊所中幽门螺杆菌菌株的抗菌耐药率和流行率很高。
