通过非放射性测定法确定干扰素-γ和细胞外色氨酸对T24细胞中吲哚胺2,3-双加氧酶活性的影响。
Influence of interferon-gamma and extracellular tryptophan on indoleamine 2,3-dioxygenase activity in T24 cells as determined by a non-radiometric assay.
作者信息
Werner E R, Werner-Felmayer G, Fuchs D, Hausen A, Reibnegger G, Wachter H
机构信息
Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Austria.
出版信息
Biochem J. 1988 Dec 1;256(2):537-41. doi: 10.1042/bj2560537.
Abstract
The indoleamine 2,3-dioxygenase (EC 1.13.11.17) activity in human T24 cells has been investigated in cell extracts by using a non-radioactive assay. It is enhanced in a dose-dependent manner up to 25-fold by interferon-gamma. The maximum reaction velocity is increased rather than the Km, which remains at 4 mumol/l. Induction of activity starts 3 h after stimulation and reaches a plateau at 21-48 h. Decreased stimulation was observed in the presence of high L-tryptophan concentrations.
摘要
已通过非放射性测定法对人T24细胞提取物中的吲哚胺2,3-双加氧酶（EC 1.13.11.17）活性进行了研究。干扰素-γ以剂量依赖方式将其增强至25倍。最大反应速度增加，而Km保持在4 μmol/l不变。活性诱导在刺激后3小时开始，并在21-48小时达到平台期。在高L-色氨酸浓度存在下观察到刺激作用减弱。
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本文引用的文献
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