Inner Ear Research Laboratory, Department of Biomedical Research (DBMR), University of Bern, Bern 3008, Switzerland
Department of Otorhinolaryngology, Head & Neck Surgery, Inselspital, Bern University Hospital, University of Bern, Bern 3010, Switzerland.
Development. 2019 Sep 2;146(17):dev177188. doi: 10.1242/dev.177188.
The development of therapeutic interventions for hearing loss requires fundamental knowledge about the signaling pathways controlling tissue development as well as the establishment of human cell-based assays to validate therapeutic strategies Recent advances in the field of stem cell biology and organoid culture systems allow the expansion and differentiation of tissue-specific progenitors and pluripotent stem cells into functional hair cells and otic-like neurons. We discuss how inner ear organoids have been developed and how they offer for the first time the opportunity to validate drug-based therapies, gene-targeting approaches and cell replacement strategies.
治疗听力损失的干预措施的发展需要关于控制组织发育的信号通路的基本知识，以及建立基于人类细胞的测定法来验证治疗策略。干细胞生物学和类器官培养系统领域的最新进展使得组织特异性祖细胞和多能干细胞能够扩增和分化为功能性毛细胞和耳原性神经元。我们讨论了内耳类器官的开发方式，以及它们如何首次提供验证基于药物的治疗方法、基因靶向方法和细胞替代策略的机会。
